@inproceedings{43413d6c117e4a8f8c776f81347cda60,
title = "Fluorescent nanodiamond - A novel nanomaterial for in vivo applications",
abstract = "Fluorescent nanodiamonds (FNDs) with a size in the range of 10-100 nm have been produced by ion irradiation and annealing, and isolated by differential centrifugation. Single particle spectroscopic characterization with confocal fluorescence microscopy and fluorescence correlation spectroscopy indicates that they are photostable and useful as an alternative to far-red fluorescent proteins for bioimaging applications. We demonstrate the application by performing in vivo imaging of bare and bioconjugated FND particles (100 nm in diameter) in C. elegans and zebrafishes and exploring the interactions between this novel nanomaterial and the model organisms. Our results indicate that FNDs can be delivered to the embryos of both organisms by microinjection and eventually into the hatched larvae in the next generation. No deleterious effects have been observed for the carbon-based nanoparticles in vivo. The high fluorescence brightness, excellent photostability, and nontoxic nature of the nanomaterial have allowed longterm imaging and tracking of embryogenesis in the organisms.",
author = "Nitin Mohan and Bailin Zhang and Chang, {Cheng Chun} and Liling Yang and Chen, {Chao Sheng} and Fang, {Chia Yi} and Hsieh, {Hsiao Han} and Cho, {Chun Yi} and Wu, {Yi Chun} and Weng, {Jui Hsia} and Chung, {Bon Chu} and Chang, {Huan Cheng}",
year = "2011",
doi = "10.1557/opl.2011.1018",
language = "English",
isbn = "9781618395610",
series = "Materials Research Society Symposium Proceedings",
pages = "25--35",
booktitle = "Carbon Functional Interfaces",
note = "2011 MRS Spring Meeting ; Conference date: 25-04-2011 Through 29-04-2011",
}