Abstract
Fluorescent nanodiamonds (FNDs) with a size in the range of 10-100 nm have been produced by ion irradiation and annealing, and isolated by differential centrifugation. Single particle spectroscopic characterization with confocal fluorescence microscopy and fluorescence correlation spectroscopy indicates that they are photostable and useful as an alternative to far-red fluorescent proteins for bioimaging applications. We demonstrate the application by performing in vivo imaging of bare and bioconjugated FND particles (100 nm in diameter) in C. elegans and zebrafishes and exploring the interactions between this novel nanomaterial and the model organisms. Our results indicate that FNDs can be delivered to the embryos of both organisms by microinjection and eventually into the hatched larvae in the next generation. No deleterious effects have been observed for the carbon-based nanoparticles in vivo. The high fluorescence brightness, excellent photostability, and nontoxic nature of the nanomaterial have allowed longterm imaging and tracking of embryogenesis in the organisms.
| Original language | English |
|---|---|
| Title of host publication | Carbon Functional Interfaces |
| Pages | 25-35 |
| Number of pages | 11 |
| DOIs | |
| Publication status | Published - Dec 1 2011 |
| Event | 2011 MRS Spring Meeting - San Francisco, CA, United States Duration: Apr 25 2011 → Apr 29 2011 |
Publication series
| Name | Materials Research Society Symposium Proceedings |
|---|---|
| Volume | 1362 |
| ISSN (Print) | 0272-9172 |
Other
| Other | 2011 MRS Spring Meeting |
|---|---|
| Country | United States |
| City | San Francisco, CA |
| Period | 4/25/11 → 4/29/11 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Condensed Matter Physics
- Mechanics of Materials
- Mechanical Engineering
Cite this
Fluorescent nanodiamond - A novel nanomaterial for in vivo applications. / Mohan, Nitin; Zhang, Bailin; Chang, Cheng Chun; Yang, Liling; Chen, Chao Sheng; Fang, Chia Yi; Hsieh, Hsiao Han; Cho, Chun Yi; Wu, Yi Chun; Weng, Jui Hsia; Chung, Bon Chu; Chang, Huan Cheng.
Carbon Functional Interfaces. 2011. p. 25-35 (Materials Research Society Symposium Proceedings; Vol. 1362).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Fluorescent nanodiamond - A novel nanomaterial for in vivo applications
AU - Mohan, Nitin
AU - Zhang, Bailin
AU - Chang, Cheng Chun
AU - Yang, Liling
AU - Chen, Chao Sheng
AU - Fang, Chia Yi
AU - Hsieh, Hsiao Han
AU - Cho, Chun Yi
AU - Wu, Yi Chun
AU - Weng, Jui Hsia
AU - Chung, Bon Chu
AU - Chang, Huan Cheng
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Fluorescent nanodiamonds (FNDs) with a size in the range of 10-100 nm have been produced by ion irradiation and annealing, and isolated by differential centrifugation. Single particle spectroscopic characterization with confocal fluorescence microscopy and fluorescence correlation spectroscopy indicates that they are photostable and useful as an alternative to far-red fluorescent proteins for bioimaging applications. We demonstrate the application by performing in vivo imaging of bare and bioconjugated FND particles (100 nm in diameter) in C. elegans and zebrafishes and exploring the interactions between this novel nanomaterial and the model organisms. Our results indicate that FNDs can be delivered to the embryos of both organisms by microinjection and eventually into the hatched larvae in the next generation. No deleterious effects have been observed for the carbon-based nanoparticles in vivo. The high fluorescence brightness, excellent photostability, and nontoxic nature of the nanomaterial have allowed longterm imaging and tracking of embryogenesis in the organisms.
AB - Fluorescent nanodiamonds (FNDs) with a size in the range of 10-100 nm have been produced by ion irradiation and annealing, and isolated by differential centrifugation. Single particle spectroscopic characterization with confocal fluorescence microscopy and fluorescence correlation spectroscopy indicates that they are photostable and useful as an alternative to far-red fluorescent proteins for bioimaging applications. We demonstrate the application by performing in vivo imaging of bare and bioconjugated FND particles (100 nm in diameter) in C. elegans and zebrafishes and exploring the interactions between this novel nanomaterial and the model organisms. Our results indicate that FNDs can be delivered to the embryos of both organisms by microinjection and eventually into the hatched larvae in the next generation. No deleterious effects have been observed for the carbon-based nanoparticles in vivo. The high fluorescence brightness, excellent photostability, and nontoxic nature of the nanomaterial have allowed longterm imaging and tracking of embryogenesis in the organisms.
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UR - http://www.scopus.com/inward/citedby.url?scp=84860166835&partnerID=8YFLogxK
U2 - 10.1557/opl.2011.1018
DO - 10.1557/opl.2011.1018
M3 - Conference contribution
AN - SCOPUS:84860166835
SN - 9781618395610
T3 - Materials Research Society Symposium Proceedings
SP - 25
EP - 35
BT - Carbon Functional Interfaces
ER -