Fluorescent polyion complex nanoparticle that incorporates an internal standard for quantitative analysis of protein kinase activity

Takanobu Nobori, Shujiro Shiosaki, Takeshi Mori, Riki Toita, Chan Woo Kim, Yuta Nakamura, Akihiro Kishimura, Takuro Niidome, Yoshiki Katayama

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We demonstrate a polyion complex (PIC) nanoparticle that contains both a responsive fluorophore and an "internal standard" fluorophore for quantitative measurement of protein kinase (PK) activity. The PK-responsive fluorophore becomes more fluorescent with PK-catalyzed phosphorylation of substrate peptides incorporated in the PIC, while fluorescence from the internal standard remains unchanged during phosphorylation. This new concept will be useful for quantitative PK assays and the discovery of PK inhibitors.

Original languageEnglish
Pages (from-to)869-872
Number of pages4
JournalBioconjugate Chemistry
Volume25
Issue number5
DOIs
Publication statusPublished - May 21 2014

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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