Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases

Yasuto Murayama, Yumiko Kurokawa, Kouta Mayanagi, Hiroshi Iwasaki

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Holliday junctions (HJs) are key intermediates in homologous recombination and are especially important for the production of crossover recombinants. Bacterial RecA family proteins promote the formation and branch migration of HJs in vitro by catalysing a reciprocal DNA-strand exchange reaction between two duplex DNA molecules, one of which contains a single-stranded DNA region that is essential for initial nucleoprotein filament formation. This activity has been reported only for prokaryotic RecA family recombinases, although eukaryotic homologues are also essential for HJ production in vivo. Here we show that fission yeast (Rhp51) and human (hRad51) RecA homologues promote duplex-duplex DNA-strand exchange in vitro. As with RecA, a HJ is formed between the two duplex DNA molecules, and reciprocal strand exchange proceeds through branch migration of the HJ. In contrast to RecA, however, strand exchange mediated by eukaryotic recombinases proceeds in the 3′→5′ direction relative to the single-stranded DNA region of the substrate DNA. The opposite polarity of Rhp51 makes it especially suitable for the repair of DNA double-strand breaks, whose repair is initiated at the processed ends of breaks that have protruding 3′ termini.

Original languageEnglish
Pages (from-to)1018-1021
Number of pages4
JournalNature
Volume451
Issue number7181
DOIs
Publication statusPublished - Feb 21 2008
Externally publishedYes

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Cruciform DNA
Recombinases
DNA
Single-Stranded DNA
Rec A Recombinases
Nucleoproteins
Double-Stranded DNA Breaks
Schizosaccharomyces
Homologous Recombination

All Science Journal Classification (ASJC) codes

  • General

Cite this

Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases. / Murayama, Yasuto; Kurokawa, Yumiko; Mayanagi, Kouta; Iwasaki, Hiroshi.

In: Nature, Vol. 451, No. 7181, 21.02.2008, p. 1018-1021.

Research output: Contribution to journalArticle

Murayama, Yasuto ; Kurokawa, Yumiko ; Mayanagi, Kouta ; Iwasaki, Hiroshi. / Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases. In: Nature. 2008 ; Vol. 451, No. 7181. pp. 1018-1021.
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