Formation and Characteristics of an Intermediate Complex EAC 1,4,2 in Immune Hemolysis by Carp Complement*1

Tomoki Yano, Hiroko Matsuyama, Miki Nakao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A hemolytic intermediate complex EAC1,4, 2 was prepared by incubating carp EAC1, 4 with hydrazine-treated carp serum, which is deficient in C4 and C3, at 0°C for 20 min. The difference between EACl, 4, 2 and EACl, 4 was shown by tests with inactivated carp sera. The former was hemolyzed by carrageenan-treated serum (Cn), ammonia-treated serum (Am) and heat-treated serum (H), whereas the latter was hemolyzed by Cn and Am, but not by H. EACl, 4,2 was unstable. Its half-life was about 15 min at 20°C and about 2.5 h at 0°C. Addition of Ca2+ to the buffer was not effective in preventing the deterioration of this complex. The reaction between EACl, 4 and Cy’ (a mixture of complement components other than Cl and C4) proceeded only in the presence of Mg2+, whereas the reaction of EACl, 4,2 with Cy’ proceeded without Mg2+. This indicates that the action of C2 requires Mg2+ while C3 acts without Mg,2 +.

Original languageEnglish
Pages (from-to)1997-2000
Number of pages4
JournalNippon Suisan Gakkaishi (Japanese Edition)
Volume54
Issue number11
DOIs
Publication statusPublished - Jan 1 1988

Fingerprint

hemolysis
carp
serum
complement
hydrazine
carrageenan
half life
ammonia
buffers
deterioration
heat
calcium
testing

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

Formation and Characteristics of an Intermediate Complex EAC 1,4,2 in Immune Hemolysis by Carp Complement*1. / Yano, Tomoki; Matsuyama, Hiroko; Nakao, Miki.

In: Nippon Suisan Gakkaishi (Japanese Edition), Vol. 54, No. 11, 01.01.1988, p. 1997-2000.

Research output: Contribution to journalArticle

@article{f3fa7c1fc8a442308daf19bda589196a,
title = "Formation and Characteristics of an Intermediate Complex EAC 1,4,2 in Immune Hemolysis by Carp Complement*1",
abstract = "A hemolytic intermediate complex EAC1,4, 2 was prepared by incubating carp EAC1, 4 with hydrazine-treated carp serum, which is deficient in C4 and C3, at 0°C for 20 min. The difference between EACl, 4, 2 and EACl, 4 was shown by tests with inactivated carp sera. The former was hemolyzed by carrageenan-treated serum (Cn), ammonia-treated serum (Am) and heat-treated serum (H), whereas the latter was hemolyzed by Cn and Am, but not by H. EACl, 4,2 was unstable. Its half-life was about 15 min at 20°C and about 2.5 h at 0°C. Addition of Ca2+ to the buffer was not effective in preventing the deterioration of this complex. The reaction between EACl, 4 and Cy’ (a mixture of complement components other than Cl and C4) proceeded only in the presence of Mg2+, whereas the reaction of EACl, 4,2 with Cy’ proceeded without Mg2+. This indicates that the action of C2 requires Mg2+ while C3 acts without Mg,2 +.",
author = "Tomoki Yano and Hiroko Matsuyama and Miki Nakao",
year = "1988",
month = "1",
day = "1",
doi = "10.2331/suisan.54.1997",
language = "English",
volume = "54",
pages = "1997--2000",
journal = "Nippon Suisan Gakkaishi",
issn = "0021-5392",
publisher = "Nihon Suisan Gakkai",
number = "11",

}

TY - JOUR

T1 - Formation and Characteristics of an Intermediate Complex EAC 1,4,2 in Immune Hemolysis by Carp Complement*1

AU - Yano, Tomoki

AU - Matsuyama, Hiroko

AU - Nakao, Miki

PY - 1988/1/1

Y1 - 1988/1/1

N2 - A hemolytic intermediate complex EAC1,4, 2 was prepared by incubating carp EAC1, 4 with hydrazine-treated carp serum, which is deficient in C4 and C3, at 0°C for 20 min. The difference between EACl, 4, 2 and EACl, 4 was shown by tests with inactivated carp sera. The former was hemolyzed by carrageenan-treated serum (Cn), ammonia-treated serum (Am) and heat-treated serum (H), whereas the latter was hemolyzed by Cn and Am, but not by H. EACl, 4,2 was unstable. Its half-life was about 15 min at 20°C and about 2.5 h at 0°C. Addition of Ca2+ to the buffer was not effective in preventing the deterioration of this complex. The reaction between EACl, 4 and Cy’ (a mixture of complement components other than Cl and C4) proceeded only in the presence of Mg2+, whereas the reaction of EACl, 4,2 with Cy’ proceeded without Mg2+. This indicates that the action of C2 requires Mg2+ while C3 acts without Mg,2 +.

AB - A hemolytic intermediate complex EAC1,4, 2 was prepared by incubating carp EAC1, 4 with hydrazine-treated carp serum, which is deficient in C4 and C3, at 0°C for 20 min. The difference between EACl, 4, 2 and EACl, 4 was shown by tests with inactivated carp sera. The former was hemolyzed by carrageenan-treated serum (Cn), ammonia-treated serum (Am) and heat-treated serum (H), whereas the latter was hemolyzed by Cn and Am, but not by H. EACl, 4,2 was unstable. Its half-life was about 15 min at 20°C and about 2.5 h at 0°C. Addition of Ca2+ to the buffer was not effective in preventing the deterioration of this complex. The reaction between EACl, 4 and Cy’ (a mixture of complement components other than Cl and C4) proceeded only in the presence of Mg2+, whereas the reaction of EACl, 4,2 with Cy’ proceeded without Mg2+. This indicates that the action of C2 requires Mg2+ while C3 acts without Mg,2 +.

UR - http://www.scopus.com/inward/record.url?scp=22944464998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22944464998&partnerID=8YFLogxK

U2 - 10.2331/suisan.54.1997

DO - 10.2331/suisan.54.1997

M3 - Article

AN - SCOPUS:22944464998

VL - 54

SP - 1997

EP - 2000

JO - Nippon Suisan Gakkaishi

JF - Nippon Suisan Gakkaishi

SN - 0021-5392

IS - 11

ER -