Formation of a monoclonal antibody against glycyrrhizin and development of an ELISA

Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization-tof mass spectrometry. Hybridomas secreting monoclonal antibody against glycyrrhizin were produced by fusing splenocytes immunized with a glycyrrhizin-bovine serum albumin conjugate with the HAT-sensitive mouse myeloma cell line P3- X63-Ag8-653. A very small cross-reaction appeared with glycyrrhetic acid monoglucuronide and glycyrrhetic acid. The full measuring range of the assay extends from 20 ng ml-1 to 200 ng ml-1 of glycyrrhizin.

Original languageEnglish
Pages (from-to)1391-1393
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume21
Issue number12
DOIs
Publication statusPublished - Jan 1 1998

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Glycyrrhizic Acid
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Bovine Serum Albumin
Glycyrrhetinic Acid
Haptens
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Cross Reactions
Hybridomas
Antigens
Cell Line

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Formation of a monoclonal antibody against glycyrrhizin and development of an ELISA. / Tanaka, Hiroyuki; Shoyama, Yukihiro.

In: Biological and Pharmaceutical Bulletin, Vol. 21, No. 12, 01.01.1998, p. 1391-1393.

Research output: Contribution to journalArticle

Tanaka, Hiroyuki ; Shoyama, Yukihiro. / Formation of a monoclonal antibody against glycyrrhizin and development of an ELISA. In: Biological and Pharmaceutical Bulletin. 1998 ; Vol. 21, No. 12. pp. 1391-1393.
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