Formation of a novel 20-hydroxylated metabolite of lipoxin A4 by human neutrophil microsomes

Hideki Sumimoto, Ryuichi Isobe, Yoichi Mizukami, Shigeki Minakami

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Lipoxin A4 (LXA4) is a biologically active compound produced from arachidonic acid via interactions of lipoxygenases. Incubation of LXA4 either with human neutrophils or with the neutrophil microsomes leads to formation of a polar compound on a reverse-phase high-performance liquid chromatography. We have identified the metabolite as 20-hydroxy-LXA4, a novel metabolite of arachidonic acid, on the basis of ultraviolet spectrometry and gas chromatography-mass spectrometry. The LXA4ω -hydroxylation requires both molecular oxygen and NADPH, and is inhibited by carbon monoxide, by antibodies raised against NADPH-cytochrome P-450 reductase, or competitively by leukotriene B4 (LTB4) and LTB5, substrates of LTB4 ω-hydroxylase. These findings indicate that the formation of 20-hydroxy-LXA4 is catalyzed by a neutrophil cytochrome P-450, the LTB4 ω-hydroxylase.

Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalFEBS Letters
Volume315
Issue number3
DOIs
Publication statusPublished - Jan 11 1993

Fingerprint

Metabolites
Microsomes
Neutrophils
Leukotriene B4
Mixed Function Oxygenases
Arachidonic Acid
Lipoxygenases
NADPH-Ferrihemoprotein Reductase
Hydroxylation
Molecular oxygen
High performance liquid chromatography
Reverse-Phase Chromatography
Carbon Monoxide
NADP
Gas chromatography
Gas Chromatography-Mass Spectrometry
Cytochrome P-450 Enzyme System
Spectrometry
Mass spectrometry
Spectrum Analysis

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Formation of a novel 20-hydroxylated metabolite of lipoxin A4 by human neutrophil microsomes. / Sumimoto, Hideki; Isobe, Ryuichi; Mizukami, Yoichi; Minakami, Shigeki.

In: FEBS Letters, Vol. 315, No. 3, 11.01.1993, p. 205-210.

Research output: Contribution to journalArticle

Sumimoto, Hideki ; Isobe, Ryuichi ; Mizukami, Yoichi ; Minakami, Shigeki. / Formation of a novel 20-hydroxylated metabolite of lipoxin A4 by human neutrophil microsomes. In: FEBS Letters. 1993 ; Vol. 315, No. 3. pp. 205-210.
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