Formation of covellite (CuS) under biological sulfate-reducing conditions

Jonathan P. Gramp, Keiko Sasaki, Jerry M. Bigham, Olia V. Karnachuk, Olli H. Tuovinen

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Sulfate-reducing bacteria (SRB) play a major role in the precipitation of metal sulfides in the environment. In this work, biogenic copper sulfide formation was examined in cultures of SRB and compared to chemically initiated Cu sulfide precipitation as a reference system. Mixed cultures of SRB were incubated at 22, 45, and 60°C in nutrient solutions that contained copper sulfate. Abiotic reference samples were produced by reacting uninoculated liquid media with Na2S solutions under otherwise identical conditions. Precipitates were collected anaerobically by centrifugation, frozen in liquid N2, and freeze-dried, followed by analysis using X-ray diffraction (XRD), X-ray fluorescence, and scanning electron microscopy. Covellite (CuS) was the only mineral found in the precipitates. Covellite was less crystalline in the biogenic precipitates than in the abiotic samples based on XRD peak widths and peak to background ratios. Poor crystallinity may be the result of slower precipitation rates in bacterial cultures as compared to the abiotic reference systems. Furthermore, bacterial cells may inhibit the nucleation steps that lead to crystal formation. Incubation at elevated temperatures improved the crystallinity of the biotic specimens.

Original languageEnglish
Pages (from-to)613-619
Number of pages7
JournalGeomicrobiology Journal
Volume23
Issue number8
DOIs
Publication statusPublished - Dec 1 2006

Fingerprint

sulfate-reducing bacterium
Sulfides
Sulfates
Precipitates
Bacteria
sulfide
crystallinity
sulfate
X-Ray Diffraction
X-ray diffraction
copper
Copper Sulfate
reference sample
liquid
Centrifugation
Liquids
X-ray fluorescence
Electron Scanning Microscopy
X ray diffraction analysis
nucleation

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Environmental Chemistry
  • Environmental Science(all)
  • Earth and Planetary Sciences (miscellaneous)

Cite this

Formation of covellite (CuS) under biological sulfate-reducing conditions. / Gramp, Jonathan P.; Sasaki, Keiko; Bigham, Jerry M.; Karnachuk, Olia V.; Tuovinen, Olli H.

In: Geomicrobiology Journal, Vol. 23, No. 8, 01.12.2006, p. 613-619.

Research output: Contribution to journalArticle

Gramp, Jonathan P. ; Sasaki, Keiko ; Bigham, Jerry M. ; Karnachuk, Olia V. ; Tuovinen, Olli H. / Formation of covellite (CuS) under biological sulfate-reducing conditions. In: Geomicrobiology Journal. 2006 ; Vol. 23, No. 8. pp. 613-619.
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