Abstract-Ginsenoside Rbl was conjugated with bovine serum albumin by NaIO4. MALDI/MS spectrum of the conjugates revealed that most abundant conjugate had 10.2 moles of hapten(ginsenoside) and hapten number ranged from 0.6 moles to 24.6 moles. Ginsenoside-protein conjugate formation was also proved using ginsenoside Rc-HSA conjugate by PAGE gelelectrophoresis. With mercaptoethanol hapten number of conjugate seemed increased but solubility decreased. Monoclonal antibody formation is under investigation with ginsenoside-BSA conjugate.
All Science Journal Classification (ASJC) codes
- Plant Science