TY - JOUR
T1 - Formation of ginsenoside-protein conjugates
AU - Park, Hoon
AU - Shoyama, Yukihiro
AU - Xuan, Li jiang
AU - Tanaka, Hiroyuki
N1 - Funding Information:
Acknowledgments--Senior author thanks Korea Science and Engineering Foundation(KOSEF) for visiting research grant for this investigation.
PY - 1999
Y1 - 1999
N2 - Abstract-Ginsenoside Rbl was conjugated with bovine serum albumin by NaIO4. MALDI/MS spectrum of the conjugates revealed that most abundant conjugate had 10.2 moles of hapten(ginsenoside) and hapten number ranged from 0.6 moles to 24.6 moles. Ginsenoside-protein conjugate formation was also proved using ginsenoside Rc-HSA conjugate by PAGE gelelectrophoresis. With mercaptoethanol hapten number of conjugate seemed increased but solubility decreased. Monoclonal antibody formation is under investigation with ginsenoside-BSA conjugate.
AB - Abstract-Ginsenoside Rbl was conjugated with bovine serum albumin by NaIO4. MALDI/MS spectrum of the conjugates revealed that most abundant conjugate had 10.2 moles of hapten(ginsenoside) and hapten number ranged from 0.6 moles to 24.6 moles. Ginsenoside-protein conjugate formation was also proved using ginsenoside Rc-HSA conjugate by PAGE gelelectrophoresis. With mercaptoethanol hapten number of conjugate seemed increased but solubility decreased. Monoclonal antibody formation is under investigation with ginsenoside-BSA conjugate.
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U2 - 10.1016/S0928-3420(99)80011-1
DO - 10.1016/S0928-3420(99)80011-1
M3 - Article
AN - SCOPUS:77957057740
VL - 6
SP - 83
EP - 86
JO - Studies in Plant Science
JF - Studies in Plant Science
SN - 0928-3420
IS - C
ER -