Formation of leukotriene B4-coenzyme a ester by rat liver microsomes

Atsushi Yamaoka, Hideki Sumimoto, Ryuichi Isobe, Shigeki Minakami

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

When leukotriene B4 (LTB4) was incubated with rat liver microsomal fraction in the presence of coenzyme A (CoA) and ATP, a more polar product (compound I) was detected on reverse-phase high-performance liquid chromatography (RP-HPLC). The product was identified as LTB4-CoA ester on the basis of ultraviolet spectrometry, alkaline hydrolysis followed by RP-HPLC, and fast atom bombardment mass spectrometry (FAB-MS). The activity forming LTB4-CoA ester was localized in the microsomal fraction. The reaction was proportional to the concentration of the microsomal protein with an optimal pH of 7.5-8.0 and completely dependent on CoA and ATP. Palmitic acid and myristic acid significantly inhibited the formation.

Original languageEnglish
Pages (from-to)1248-1252
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume154
Issue number3
DOIs
Publication statusPublished - Aug 15 1988

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Leukotriene B4
Coenzymes
Liver Microsomes
Coenzyme A
Liver
Rats
Esters
High performance liquid chromatography
Reverse-Phase Chromatography
Adenosine Triphosphate
High Pressure Liquid Chromatography
Fast Atom Bombardment Mass Spectrometry
Palmitic Acid
Myristic Acid
Spectrometry
Mass spectrometry
Hydrolysis
Spectrum Analysis
Atoms
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Formation of leukotriene B4-coenzyme a ester by rat liver microsomes. / Yamaoka, Atsushi; Sumimoto, Hideki; Isobe, Ryuichi; Minakami, Shigeki.

In: Biochemical and Biophysical Research Communications, Vol. 154, No. 3, 15.08.1988, p. 1248-1252.

Research output: Contribution to journalArticle

Yamaoka, Atsushi ; Sumimoto, Hideki ; Isobe, Ryuichi ; Minakami, Shigeki. / Formation of leukotriene B4-coenzyme a ester by rat liver microsomes. In: Biochemical and Biophysical Research Communications. 1988 ; Vol. 154, No. 3. pp. 1248-1252.
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