TY - JOUR
T1 - Formation of leukotriene B4-coenzyme a ester by rat liver microsomes
AU - Yamaoka, Atsushi
AU - Sumimoto, Hideki
AU - Isobe, Ryuichi
AU - Minakami, Shigeki
N1 - Funding Information:
This study is supported in part by grants from the Ministry Japan.
PY - 1988/8/15
Y1 - 1988/8/15
N2 - When leukotriene B4 (LTB4) was incubated with rat liver microsomal fraction in the presence of coenzyme A (CoA) and ATP, a more polar product (compound I) was detected on reverse-phase high-performance liquid chromatography (RP-HPLC). The product was identified as LTB4-CoA ester on the basis of ultraviolet spectrometry, alkaline hydrolysis followed by RP-HPLC, and fast atom bombardment mass spectrometry (FAB-MS). The activity forming LTB4-CoA ester was localized in the microsomal fraction. The reaction was proportional to the concentration of the microsomal protein with an optimal pH of 7.5-8.0 and completely dependent on CoA and ATP. Palmitic acid and myristic acid significantly inhibited the formation.
AB - When leukotriene B4 (LTB4) was incubated with rat liver microsomal fraction in the presence of coenzyme A (CoA) and ATP, a more polar product (compound I) was detected on reverse-phase high-performance liquid chromatography (RP-HPLC). The product was identified as LTB4-CoA ester on the basis of ultraviolet spectrometry, alkaline hydrolysis followed by RP-HPLC, and fast atom bombardment mass spectrometry (FAB-MS). The activity forming LTB4-CoA ester was localized in the microsomal fraction. The reaction was proportional to the concentration of the microsomal protein with an optimal pH of 7.5-8.0 and completely dependent on CoA and ATP. Palmitic acid and myristic acid significantly inhibited the formation.
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U2 - 10.1016/0006-291X(88)90273-2
DO - 10.1016/0006-291X(88)90273-2
M3 - Article
C2 - 2841929
AN - SCOPUS:0024288494
SN - 0006-291X
VL - 154
SP - 1248
EP - 1252
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -