Formation of methyl mercaptan from L-methionine by Porphyromonas gingivalis

Mamiko Yoshimura, Yoshio Nakano, Yoshihisa Yamashita, Takahiko Oho, Toshiyuki Saito, Toshihiko Koga

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Abstract

Methyl mercaptan production by oral bacteria is thought to be one of the main causes of oral malodor. We examined the ability of periodontopathic Porphyromonas gingivalis to produce methyl mercaptan from L-methionine and found that the invasive strains W83 and W50 produced large amounts of methyl mercaptan. We cloned and sequenced the mgl gene encoding L-methionine-α-deamino-γ-mercaptomethane-lyase (METase) from P. gingivalis W83. The structural mgl gene consisted of 1,200 bp and encoded a 43.3-kDa protein. To examine the role of methyl mercaptan in the pathogenesis of P. gingivalis, a METase-deficient mutant of P. gingivalis W83 was constructed. The methionine degradation activity and virulence of the mutant (M1217) and the parent strain (W83) in mice were compared. M1217 showed a marked decrease in the formation of methyl mercaptan from L-methionine and decreased virulence compared with the wild-type strain W83. These results suggest that methyl mercaptan not only is one of the sources of oral malodor, but may also play a role in the pathogenicity of P. gingivalis.

Original languageEnglish
Pages (from-to)6912-6916
Number of pages5
JournalInfection and Immunity
Volume68
Issue number12
DOIs
Publication statusPublished - Dec 1 2000

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Porphyromonas gingivalis
Sulfhydryl Compounds
Methionine
Virulence
Lyases
Genes
Bacteria
Proteins

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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Formation of methyl mercaptan from L-methionine by Porphyromonas gingivalis. / Yoshimura, Mamiko; Nakano, Yoshio; Yamashita, Yoshihisa; Oho, Takahiko; Saito, Toshiyuki; Koga, Toshihiko.

In: Infection and Immunity, Vol. 68, No. 12, 01.12.2000, p. 6912-6916.

Research output: Contribution to journalArticle

Yoshimura, Mamiko ; Nakano, Yoshio ; Yamashita, Yoshihisa ; Oho, Takahiko ; Saito, Toshiyuki ; Koga, Toshihiko. / Formation of methyl mercaptan from L-methionine by Porphyromonas gingivalis. In: Infection and Immunity. 2000 ; Vol. 68, No. 12. pp. 6912-6916.
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