Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization

Hiroyuki Tanaka, Noriko Fukuda, Yukihiro Shoyama

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Rc and ginsenoside Rd. The full measuring range of the assay extends from 20 ngml-1 to 400 ngml-1 of ginsenoside Rb1.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalCytotechnology
Volume29
Issue number2
DOIs
Publication statusPublished - Jan 1 1999

Fingerprint

Panax
Monoclonal antibodies
Antigens
Ionization
Mass spectrometry
Assays
Desorption
Monoclonal Antibodies
Cells
Bovine Serum Albumin
Lasers
Ginsenosides
Haptens
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Cross Reactions
Hybridomas
Cell Line
ginsenoside Rb1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

Cite this

Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization. / Tanaka, Hiroyuki; Fukuda, Noriko; Shoyama, Yukihiro.

In: Cytotechnology, Vol. 29, No. 2, 01.01.1999, p. 115-120.

Research output: Contribution to journalArticle

Tanaka, Hiroyuki ; Fukuda, Noriko ; Shoyama, Yukihiro. / Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization. In: Cytotechnology. 1999 ; Vol. 29, No. 2. pp. 115-120.
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