Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization: Monoclonal antibody for a ginseng saponin

Noriko Fukuda, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rg1 was produced by fusing sprenocytes immunized with a ginsenoside Rg1-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Re. The full measuring range of the assay extends from 0.3 μg ml-1 to 10 μg ml-1 of ginsenoside Rg1.

Original languageEnglish
Pages (from-to)197-204
Number of pages8
JournalCytotechnology
Volume34
Issue number3
DOIs
Publication statusPublished - Jan 1 2000

Fingerprint

Panax
Monoclonal antibodies
Saponins
Antigens
Ionization
Mass spectrometry
Assays
Desorption
Monoclonal Antibodies
Cells
Bovine Serum Albumin
Lasers
Ginsenosides
Haptens
Cross Reactions
Hybridomas
Mass Spectrometry
Cell Line
ginsenoside Rg1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

Cite this

Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization : Monoclonal antibody for a ginseng saponin. / Fukuda, Noriko; Tanaka, Hiroyuki; Shoyama, Yukihiro.

In: Cytotechnology, Vol. 34, No. 3, 01.01.2000, p. 197-204.

Research output: Contribution to journalArticle

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