Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization: Monoclonal antibody for a ginseng saponin

Noriko Fukuda, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rg1 was produced by fusing sprenocytes immunized with a ginsenoside Rg1-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Re. The full measuring range of the assay extends from 0.3 μg ml-1 to 10 μg ml-1 of ginsenoside Rg1.

Original languageEnglish
Pages (from-to)197-204
Number of pages8
JournalCytotechnology
Volume34
Issue number3
DOIs
Publication statusPublished - Jan 1 2000

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

Cite this