The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rg1 was produced by fusing sprenocytes immunized with a ginsenoside Rg1-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Re. The full measuring range of the assay extends from 0.3 μg ml-1 to 10 μg ml-1 of ginsenoside Rg1.
All Science Journal Classification (ASJC) codes
- Biomedical Engineering
- Clinical Biochemistry
- Cell Biology