Formation of monoclonal antibody against naturally occurring biologically active compounds and their application

Hiroyuki Tanaka

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1 Citation (Scopus)

Abstract

In the course of a program in developing new enzyme-linked immunosorbent assay (ELISA)-methods for the quantification of bioactive natural products in plants, phytomedicines and animals in a μg and ng scale, monoclonal antibodies against various natural products of medicInal have been developed. The cross- reaction of anti-forskolin antibodies with 7-deacetyl forskolin was 5.6%. A very small cross-reaction appeared with other derivatives. The full measuring range of the assay extends from 5 ngml-1 to 5 μgml-1 A MAb againstΔ1-tetrahydrocannabinolic acid (THCA) was produced. AntiΔ1-THCA antibody reacted with a range of other cannabinoids, including many cannabinoids and a spiro-compound, but not with other phenolics. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 (G-Rb1) was produced, which scarcely cross-reacted with ginsenosides Rc and Rd. Immunoaffinity column chromatography using this anti-G-Rb1 MAb appeared to be far superior to previously published separation methods by crystallization and chromatography, and the capacity of the immunoaffinity column as determined by the ELISA was 20 μg/ml gel. Anti-glycyrrhizin MAbs were produced. TLC immunostaining, in which TLC-separated glycyrrhetic acid glucuronides were transferred to a polyvinylidene difluoride membrane, converted into a glycyrrhetic acid glucuronide-BSA conjugate, and immunostained, was more sensitive than other staining.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalNatural Medicines
Volume54
Issue number3
Publication statusPublished - Jun 1 2000

All Science Journal Classification (ASJC) codes

  • Molecular Medicine

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