TY - JOUR
T1 - Formation of Multi-Channel Collagen Gels Investigated Using Particle Tracking Microrheology
AU - Yonemoto, Junta
AU - Maki, Yasuyuki
AU - Koh, Isabel
AU - Furusawa, Kazuya
AU - Annaka, Masahiko
N1 - Funding Information:
This work was supported by JSPS KAKENHI (Grants-in-Aid for Scientific Research) grant numbers 15K20906 and 18K05519.
Publisher Copyright:
© 2021 American Chemical Society
PY - 2021/9/13
Y1 - 2021/9/13
N2 - Collagen is one of the most common materials used to form scaffolds for tissue engineering applications. The multi-channel collagen gel (MCCG) obtained by the dialysis of an acidic collagen solution in a neutral buffer solution has a unique structure, with many capillaries of diameters several tens to a few hundred micrometers, and could be a potential candidate as a biomimetic scaffold for three-dimensional tissue engineering. In the present study, the formation of MCCG was investigated byin siturheological measurements based on a particle tracking method (particle tracking microrheology, PTM). PTM enabled us to measure changes in the rheological properties of collagen solutions under the continuous exchange of substances during dialysis. When an observation plane was set perpendicular to the direction of gel growth, we first observed convectional flow of the collagen solution, followed by phase separation and gelation. We showed that the structure of the MCCG originated from the transient structure formed during the initial stage of viscoelastic phase separation and was fixed by the subsequent gelation.
AB - Collagen is one of the most common materials used to form scaffolds for tissue engineering applications. The multi-channel collagen gel (MCCG) obtained by the dialysis of an acidic collagen solution in a neutral buffer solution has a unique structure, with many capillaries of diameters several tens to a few hundred micrometers, and could be a potential candidate as a biomimetic scaffold for three-dimensional tissue engineering. In the present study, the formation of MCCG was investigated byin siturheological measurements based on a particle tracking method (particle tracking microrheology, PTM). PTM enabled us to measure changes in the rheological properties of collagen solutions under the continuous exchange of substances during dialysis. When an observation plane was set perpendicular to the direction of gel growth, we first observed convectional flow of the collagen solution, followed by phase separation and gelation. We showed that the structure of the MCCG originated from the transient structure formed during the initial stage of viscoelastic phase separation and was fixed by the subsequent gelation.
UR - http://www.scopus.com/inward/record.url?scp=85113170797&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85113170797&partnerID=8YFLogxK
U2 - 10.1021/acs.biomac.1c00666
DO - 10.1021/acs.biomac.1c00666
M3 - Article
C2 - 34343432
AN - SCOPUS:85113170797
VL - 22
SP - 3819
EP - 3826
JO - Biomacromolecules
JF - Biomacromolecules
SN - 1525-7797
IS - 9
ER -