Formation of nucleus-like structure in the cytoplasm of λ-DNA-injected fertilized eggs and its partition into blastomeres during early embryogenesis in Xenopus laevis

Koichiro Shiokawa, Munefumi Sameshima, Kosuke Tashiro, Takayo Miura, Norihiko Nakakura, K. Yamana

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The fate of bacteriophage λ-DNA was examined after injection into the fertilized eggs of Xenopus laevis. Injection of a large amount of λ-DNA (ca. 24 ng) into a fertilized Xenopus egg induced the formation around the injected DNA of a giant nucleus-like structure which was surrounded by an apparently normal bilayered nuclear membrane with nuclear pore complexes. Southern blot analysis revealed the persistence of injected λ-DNA until the blastula stage. The nucleus-like structure was partitioned into blastomeres during cleavage through a process of nuclear fission, and was maintained in a group of extraordinarily large blastomeres until the blastula stage.

Original languageEnglish
Pages (from-to)539-542
Number of pages4
JournalDevelopmental Biology
Volume116
Issue number2
DOIs
Publication statusPublished - Jan 1 1986

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Blastomeres
Zygote
Xenopus laevis
Embryonic Development
Cytoplasm
Blastula
DNA
Nuclear Fission
Nuclear Pore
Injections
Nuclear Envelope
Xenopus
Southern Blotting
Bacteriophages

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Formation of nucleus-like structure in the cytoplasm of λ-DNA-injected fertilized eggs and its partition into blastomeres during early embryogenesis in Xenopus laevis. / Shiokawa, Koichiro; Sameshima, Munefumi; Tashiro, Kosuke; Miura, Takayo; Nakakura, Norihiko; Yamana, K.

In: Developmental Biology, Vol. 116, No. 2, 01.01.1986, p. 539-542.

Research output: Contribution to journalArticle

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