Formation of ring-shaped assembly of microtubules with a narrow size distribution at an air-buffer interface

Arif Md Rashedul Kabir, Shoki Wada, Daisuke Inoue, Yoshiki Tamura, Tamaki Kajihara, Hiroyuki Mayama, Kazuki Sada, Akira Kakugo, Jian Ping Gong

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22 Citations (Scopus)

Abstract

Biopolymers such as actin, microtubules and DNA are well known for their fascinating in vivo self-organization phenomena. Considerable efforts have been devoted to mimicking their organization process in vitro that produced ring-shaped or toroid structures in an irreversible manner. However, understanding the factors that lead to formation of such assembled structures deserves more investigation to achieve a unified insight into the assembly process, particularly of the microtubules. Here, we report an active assembly process of microtubules (MTs) at an air-buffer interface that resulted in ring-shaped microtubule structures with a narrow size distribution and a high yield. Using an "air-buffer interface control system" combined with the newly developed "inert chamber system (ICS)" we have also successfully observed the reversible conformational transition between ring- and linear-shaped microtubules at the air-buffer interface. This is the first ever direct in situ observation of a reversible assembly process of MTs and probably provides us with valuable discernment to understand the in vivo organizational behavior of biopolymers.

Original languageEnglish
Pages (from-to)10863-10867
Number of pages5
JournalSoft Matter
Volume8
Issue number42
DOIs
Publication statusPublished - Nov 14 2012
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

Rashedul Kabir, A. M., Wada, S., Inoue, D., Tamura, Y., Kajihara, T., Mayama, H., ... Gong, J. P. (2012). Formation of ring-shaped assembly of microtubules with a narrow size distribution at an air-buffer interface. Soft Matter, 8(42), 10863-10867. https://doi.org/10.1039/c2sm26441b