Formation of ring-shaped microtubule assemblies through active self-organization on dynein

Masaki Ito, Arif Md Rashedul Kabir, Daisuke Inoue, Takayuki Torisawa, Yoko Toyoshima, Kazuki Sada, Akira Kakugo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Microtubule (MT)-kinesin, a biomolecular motor system, is a promising candidate for construction of artificial biomachines for a variety of nanotechnology applications. An active self-organization (AcSO) method involving a specific streptavidin (St)-biotin (Bt) interaction has been developed to assemble MTs into a highly ordered structure by exploiting their motility on a kinesin-coated surface. Dynein is another biomolecular motor that moves along the MTs in the opposite direction from kinesin. Dynein has not yet been used to demonstrate the AcSO of MTs. In this study, we report the first successful demonstration of the AcSO of MTs on a dynein-coated surface to produce ring-shaped MT assemblies similar to those of kinesin. We found that ring-shaped MT assemblies obtained on dynein showed equal clockwise and counterclockwise rotational motion. This work will enrich the building blocks for designing future oriented motor protein-based artificial devices.

Original languageEnglish
Pages (from-to)220-225
Number of pages6
JournalPolymer Journal
Volume46
Issue number4
DOIs
Publication statusPublished - Apr 2014
Externally publishedYes

Fingerprint

Dyneins
Kinesin
Nanotechnology
Streptavidin
Demonstrations
Biotin
Proteins

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Materials Chemistry

Cite this

Ito, M., Kabir, A. M. R., Inoue, D., Torisawa, T., Toyoshima, Y., Sada, K., & Kakugo, A. (2014). Formation of ring-shaped microtubule assemblies through active self-organization on dynein. Polymer Journal, 46(4), 220-225. https://doi.org/10.1038/pj.2013.89

Formation of ring-shaped microtubule assemblies through active self-organization on dynein. / Ito, Masaki; Kabir, Arif Md Rashedul; Inoue, Daisuke; Torisawa, Takayuki; Toyoshima, Yoko; Sada, Kazuki; Kakugo, Akira.

In: Polymer Journal, Vol. 46, No. 4, 04.2014, p. 220-225.

Research output: Contribution to journalArticle

Ito, M, Kabir, AMR, Inoue, D, Torisawa, T, Toyoshima, Y, Sada, K & Kakugo, A 2014, 'Formation of ring-shaped microtubule assemblies through active self-organization on dynein', Polymer Journal, vol. 46, no. 4, pp. 220-225. https://doi.org/10.1038/pj.2013.89
Ito, Masaki ; Kabir, Arif Md Rashedul ; Inoue, Daisuke ; Torisawa, Takayuki ; Toyoshima, Yoko ; Sada, Kazuki ; Kakugo, Akira. / Formation of ring-shaped microtubule assemblies through active self-organization on dynein. In: Polymer Journal. 2014 ; Vol. 46, No. 4. pp. 220-225.
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