Abstract
Conifer foliage-like rutile TiO2 nanoparticles (CFR NPs), spherical anatase TiO2 NPs (SA NPs), and a mixture of CFR and SA NPs were synthesized in aqueous solution at low temperature. Furthermore, sea urchin-like nanostructures combining SA and CFR NPs, which were designated as anatase/rutile nanostructures (A/R NSs), were produced through the self-assembly of jack bean urease (JBU). The specific surface area of the A/R NSs was considerably larger than those of the CFR NPs, SA NPs, and commercial TiO 2 (P25). In addition, the amount of reactive oxygen species (ROS) yielded from the A/R NSs was significantly higher than that yielded from CFR NPs, SA NPs, and P25 because of the large surface area of the A/R NSs and a synergistic effect caused by the integration of anatase and rutile phases. The A/R NSs showed no cytotoxicity at concentrations <100 μg/mL, although CFR NPs, SA NPs, and P25 were cytotoxic, probably because of their size and shape. Using the high surface area and the superior photocatalytic activity of the A/R NSs, macrophages were effectively destroyed by UV irradiation for the purpose of treating atherosclerosis. Macrophages were killed more effectively by the A/R NSs than P25. Furthermore, different mechanisms of cell destruction resulting from UV irradiation, A/R NSs, and a combination of both were investigated. The death of cells treated with A/R NSs and exposed to UV irradiation was induced primarily by apoptosis rather than necrosis; cells that were not treated with the NSs died mainly from necrosis.
| Original language | English |
|---|---|
| Pages (from-to) | 3341-3347 |
| Number of pages | 7 |
| Journal | Chemistry of Materials |
| Volume | 23 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - Jul 26 2011 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Chemical Engineering(all)
- Materials Chemistry
Cite this
Formation of TiO2 nanostructures by enzyme-mediated self-assembly for the destruction of macrophages. / Hayashi, Koichiro; Nakamura, Michihiro; Sakamoto, Wataru; Yogo, Toshinobu; Kori, Toshinari; Ishimura, Kazunori.
In: Chemistry of Materials, Vol. 23, No. 14, 26.07.2011, p. 3341-3347.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Formation of TiO2 nanostructures by enzyme-mediated self-assembly for the destruction of macrophages
AU - Hayashi, Koichiro
AU - Nakamura, Michihiro
AU - Sakamoto, Wataru
AU - Yogo, Toshinobu
AU - Kori, Toshinari
AU - Ishimura, Kazunori
PY - 2011/7/26
Y1 - 2011/7/26
N2 - Conifer foliage-like rutile TiO2 nanoparticles (CFR NPs), spherical anatase TiO2 NPs (SA NPs), and a mixture of CFR and SA NPs were synthesized in aqueous solution at low temperature. Furthermore, sea urchin-like nanostructures combining SA and CFR NPs, which were designated as anatase/rutile nanostructures (A/R NSs), were produced through the self-assembly of jack bean urease (JBU). The specific surface area of the A/R NSs was considerably larger than those of the CFR NPs, SA NPs, and commercial TiO 2 (P25). In addition, the amount of reactive oxygen species (ROS) yielded from the A/R NSs was significantly higher than that yielded from CFR NPs, SA NPs, and P25 because of the large surface area of the A/R NSs and a synergistic effect caused by the integration of anatase and rutile phases. The A/R NSs showed no cytotoxicity at concentrations <100 μg/mL, although CFR NPs, SA NPs, and P25 were cytotoxic, probably because of their size and shape. Using the high surface area and the superior photocatalytic activity of the A/R NSs, macrophages were effectively destroyed by UV irradiation for the purpose of treating atherosclerosis. Macrophages were killed more effectively by the A/R NSs than P25. Furthermore, different mechanisms of cell destruction resulting from UV irradiation, A/R NSs, and a combination of both were investigated. The death of cells treated with A/R NSs and exposed to UV irradiation was induced primarily by apoptosis rather than necrosis; cells that were not treated with the NSs died mainly from necrosis.
AB - Conifer foliage-like rutile TiO2 nanoparticles (CFR NPs), spherical anatase TiO2 NPs (SA NPs), and a mixture of CFR and SA NPs were synthesized in aqueous solution at low temperature. Furthermore, sea urchin-like nanostructures combining SA and CFR NPs, which were designated as anatase/rutile nanostructures (A/R NSs), were produced through the self-assembly of jack bean urease (JBU). The specific surface area of the A/R NSs was considerably larger than those of the CFR NPs, SA NPs, and commercial TiO 2 (P25). In addition, the amount of reactive oxygen species (ROS) yielded from the A/R NSs was significantly higher than that yielded from CFR NPs, SA NPs, and P25 because of the large surface area of the A/R NSs and a synergistic effect caused by the integration of anatase and rutile phases. The A/R NSs showed no cytotoxicity at concentrations <100 μg/mL, although CFR NPs, SA NPs, and P25 were cytotoxic, probably because of their size and shape. Using the high surface area and the superior photocatalytic activity of the A/R NSs, macrophages were effectively destroyed by UV irradiation for the purpose of treating atherosclerosis. Macrophages were killed more effectively by the A/R NSs than P25. Furthermore, different mechanisms of cell destruction resulting from UV irradiation, A/R NSs, and a combination of both were investigated. The death of cells treated with A/R NSs and exposed to UV irradiation was induced primarily by apoptosis rather than necrosis; cells that were not treated with the NSs died mainly from necrosis.
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UR - http://www.scopus.com/inward/citedby.url?scp=79960504390&partnerID=8YFLogxK
U2 - 10.1021/cm200826c
DO - 10.1021/cm200826c
M3 - Article
AN - SCOPUS:79960504390
VL - 23
SP - 3341
EP - 3347
JO - Chemistry of Materials
JF - Chemistry of Materials
SN - 0897-4756
IS - 14
ER -