FosB gene products trigger cell proliferation and morphological alteration with an increased expression of a novel processed form of galectin-1 in the rat 3Y1 embryo cell line

Tomoko Nishioka, Sakumi Kunihiko, Tomofumi Miura, Kazuki Tahara, Hidenori Horie, Toshihiko Kadoya, Yusaku Nakabeppu

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In this study, we established rat 3Y1 embryo cell lines expressing FosB and ΔFosB as fusion proteins (ER-FosB, ER-ΔFosB) with the ligand-binding domain of human estrogen receptor (ER). The binding of estrogen to the fusion proteins resulted in their nuclear translocation. After estrogen administration, exponentially growing cells expressing ER- ΔFosB, and to a lesser extent ER-FosB, underwent morphological alteration from the flat fibroblastic shape to an extended bipolar shape, and ceased proliferating. Such morphological alteration was also induced in quiescent cells expressing ER-ΔFosB and ER-FosB after one round of cell division triggered by estrogen administration. The cells expressing ER-ΔFosB changed shape frequently, and the content of F-actin in the cytoplasm detected by binding of Alexa 488-phalloidin significantly decreased after the morphological alteration. By two-dimensional gel electrophoresis analysis of cellular proteins from the cells expressing ER-ΔFosB, we identified several proteins whose expression either increased or decreased after estrogen administration. Two of these proteins were identified from their amino acid sequences as novel processed form of galectin-1.

Original languageEnglish
Pages (from-to)653-661
Number of pages9
JournalJournal of biochemistry
Volume131
Issue number5
DOIs
Publication statusPublished - Jan 1 2002

Fingerprint

Galectin 1
Cell proliferation
Estrogen Receptors
Rats
Embryonic Structures
Genes
Cells
Cell Proliferation
Cell Line
Estrogens
Proteins
Fusion reactions
Phalloidine
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Cell Division
Actins
Amino Acid Sequence
Cytoplasm
Gels

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

FosB gene products trigger cell proliferation and morphological alteration with an increased expression of a novel processed form of galectin-1 in the rat 3Y1 embryo cell line. / Nishioka, Tomoko; Kunihiko, Sakumi; Miura, Tomofumi; Tahara, Kazuki; Horie, Hidenori; Kadoya, Toshihiko; Nakabeppu, Yusaku.

In: Journal of biochemistry, Vol. 131, No. 5, 01.01.2002, p. 653-661.

Research output: Contribution to journalArticle

@article{1ef429c7df714da79a0872b021e0fef8,
title = "FosB gene products trigger cell proliferation and morphological alteration with an increased expression of a novel processed form of galectin-1 in the rat 3Y1 embryo cell line",
abstract = "In this study, we established rat 3Y1 embryo cell lines expressing FosB and ΔFosB as fusion proteins (ER-FosB, ER-ΔFosB) with the ligand-binding domain of human estrogen receptor (ER). The binding of estrogen to the fusion proteins resulted in their nuclear translocation. After estrogen administration, exponentially growing cells expressing ER- ΔFosB, and to a lesser extent ER-FosB, underwent morphological alteration from the flat fibroblastic shape to an extended bipolar shape, and ceased proliferating. Such morphological alteration was also induced in quiescent cells expressing ER-ΔFosB and ER-FosB after one round of cell division triggered by estrogen administration. The cells expressing ER-ΔFosB changed shape frequently, and the content of F-actin in the cytoplasm detected by binding of Alexa 488-phalloidin significantly decreased after the morphological alteration. By two-dimensional gel electrophoresis analysis of cellular proteins from the cells expressing ER-ΔFosB, we identified several proteins whose expression either increased or decreased after estrogen administration. Two of these proteins were identified from their amino acid sequences as novel processed form of galectin-1.",
author = "Tomoko Nishioka and Sakumi Kunihiko and Tomofumi Miura and Kazuki Tahara and Hidenori Horie and Toshihiko Kadoya and Yusaku Nakabeppu",
year = "2002",
month = "1",
day = "1",
doi = "10.1093/oxfordjournals.jbchem.a003148",
language = "English",
volume = "131",
pages = "653--661",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - FosB gene products trigger cell proliferation and morphological alteration with an increased expression of a novel processed form of galectin-1 in the rat 3Y1 embryo cell line

AU - Nishioka, Tomoko

AU - Kunihiko, Sakumi

AU - Miura, Tomofumi

AU - Tahara, Kazuki

AU - Horie, Hidenori

AU - Kadoya, Toshihiko

AU - Nakabeppu, Yusaku

PY - 2002/1/1

Y1 - 2002/1/1

N2 - In this study, we established rat 3Y1 embryo cell lines expressing FosB and ΔFosB as fusion proteins (ER-FosB, ER-ΔFosB) with the ligand-binding domain of human estrogen receptor (ER). The binding of estrogen to the fusion proteins resulted in their nuclear translocation. After estrogen administration, exponentially growing cells expressing ER- ΔFosB, and to a lesser extent ER-FosB, underwent morphological alteration from the flat fibroblastic shape to an extended bipolar shape, and ceased proliferating. Such morphological alteration was also induced in quiescent cells expressing ER-ΔFosB and ER-FosB after one round of cell division triggered by estrogen administration. The cells expressing ER-ΔFosB changed shape frequently, and the content of F-actin in the cytoplasm detected by binding of Alexa 488-phalloidin significantly decreased after the morphological alteration. By two-dimensional gel electrophoresis analysis of cellular proteins from the cells expressing ER-ΔFosB, we identified several proteins whose expression either increased or decreased after estrogen administration. Two of these proteins were identified from their amino acid sequences as novel processed form of galectin-1.

AB - In this study, we established rat 3Y1 embryo cell lines expressing FosB and ΔFosB as fusion proteins (ER-FosB, ER-ΔFosB) with the ligand-binding domain of human estrogen receptor (ER). The binding of estrogen to the fusion proteins resulted in their nuclear translocation. After estrogen administration, exponentially growing cells expressing ER- ΔFosB, and to a lesser extent ER-FosB, underwent morphological alteration from the flat fibroblastic shape to an extended bipolar shape, and ceased proliferating. Such morphological alteration was also induced in quiescent cells expressing ER-ΔFosB and ER-FosB after one round of cell division triggered by estrogen administration. The cells expressing ER-ΔFosB changed shape frequently, and the content of F-actin in the cytoplasm detected by binding of Alexa 488-phalloidin significantly decreased after the morphological alteration. By two-dimensional gel electrophoresis analysis of cellular proteins from the cells expressing ER-ΔFosB, we identified several proteins whose expression either increased or decreased after estrogen administration. Two of these proteins were identified from their amino acid sequences as novel processed form of galectin-1.

UR - http://www.scopus.com/inward/record.url?scp=0036560930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036560930&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.jbchem.a003148

DO - 10.1093/oxfordjournals.jbchem.a003148

M3 - Article

C2 - 11983071

AN - SCOPUS:0036560930

VL - 131

SP - 653

EP - 661

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 5

ER -