TY - JOUR
T1 - Free radical imaging of endogenous redox molecules using dynamic nuclear polarisation magnetic resonance imaging
AU - Hyodo, Fuminori
AU - Ito, Shinji
AU - Eto, Hinako
AU - Elhelaly, Abdelazim Elsayed
AU - Murata, Masaharu
AU - Akahoshi, Tomohiko
AU - Utsumi, Hideo
AU - Matuso, Masayuki
N1 - Publisher Copyright:
© 2020 Informa UK Limited, trading as Taylor & Francis Group.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - Redox reactions accompanied by the oxidation–reduction of endogenous molecules play important roles in maintaining homeostasis in living organisms. In humans, numerous endogenous molecules that contribute towards maintaining physiological conditions form free radicals via electron transfer. A typical example of this is the mitochondrial electron transport chain, which is involved in energy production. If free radicals derived from endogenous molecules could be visualised and exploited as biological and functional probes, redox reactions mediated by endogenous molecules could be detected non-invasively. We succeeded in visualising the free radicals derived from endogenous molecules using an in vivo dynamic nuclear polarisation (DNP) magnetic resonance imaging (MRI) system. In this review, we describe the visualisation of endogenous redox molecules, such as flavins and ubiquinones, which are mitochondrial electron carriers, as well as vitamin E and vitamin C (ascorbate). In addition, we describe the application of melanin free radicals for the in vivo visualisation of metabola without using probes via in vivo DNP-MRI.
AB - Redox reactions accompanied by the oxidation–reduction of endogenous molecules play important roles in maintaining homeostasis in living organisms. In humans, numerous endogenous molecules that contribute towards maintaining physiological conditions form free radicals via electron transfer. A typical example of this is the mitochondrial electron transport chain, which is involved in energy production. If free radicals derived from endogenous molecules could be visualised and exploited as biological and functional probes, redox reactions mediated by endogenous molecules could be detected non-invasively. We succeeded in visualising the free radicals derived from endogenous molecules using an in vivo dynamic nuclear polarisation (DNP) magnetic resonance imaging (MRI) system. In this review, we describe the visualisation of endogenous redox molecules, such as flavins and ubiquinones, which are mitochondrial electron carriers, as well as vitamin E and vitamin C (ascorbate). In addition, we describe the application of melanin free radicals for the in vivo visualisation of metabola without using probes via in vivo DNP-MRI.
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U2 - 10.1080/10715762.2020.1859109
DO - 10.1080/10715762.2020.1859109
M3 - Review article
AN - SCOPUS:85097487519
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
ER -