Free Radical Imaging Using in Vivo Dynamic Nuclear Polarization-MRI

Hideo Utsumi, Fuminori Hyodo

    Research output: Chapter in Book/Report/Conference proceedingChapter

    10 Citations (Scopus)

    Abstract

    Redox reactions that generate free radical intermediates are essential to metabolic processes, and their intermediates can produce reactive oxygen species, which may promote diseases related to oxidative stress. The development of an in vivo electron spin resonance (ESR) spectrometer and its imaging enables us noninvasive and direct measurement of in vivo free radical reactions in living organisms. The dynamic nuclear polarization magnetic resonance imaging (DNP-MRI), also called PEDRI or OMRI, is also a new imaging method for observing free radical species in vivo. The spatiotemporal resolution of free radical imaging with DNP-MRI is comparable with that in MRI, and each of the radical species can be distinguished in the spectroscopic images by changing the frequency or magnetic field of ESR irradiation. Several kinds of stable nitroxyl radicals were used as spin probes to detect in vivo redox reactions. The signal decay of nitroxyl probes, which is determined with in vivo DNP-MRI, reflects the redox status under oxidative stress, and the signal decay is suppressed by prior administration of antioxidants. In addition, DNP-MRI can also visualize various intermediate free radicals from the intrinsic redox molecules. This noninvasive method, in vivo DNP-MRI, could become a useful tool for investigating the mechanism of oxidative injuries in animal disease models and the in vivo effects of antioxidant drugs.

    Original languageEnglish
    Title of host publicationMethods in Enzymology
    PublisherAcademic Press Inc.
    Pages553-571
    Number of pages19
    DOIs
    Publication statusPublished - 2015

    Publication series

    NameMethods in Enzymology
    Volume564
    ISSN (Print)0076-6879
    ISSN (Electronic)1557-7988

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Molecular Biology

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