Two fatty acid desaturases were independently expressed in tobacco plants and freezing tolerance of the transgenic plants was investigated: the two desaturases were CvFAD2, which desaturates microsomal C18:1 (oleic acid) to C18:2 (linoleic acid), and CvFAD3, which desaturates microsomal C18:2 to C18:3 (linolenic acid). Fatty acid composition analysis showed that the C18:2 contents in the plants transformed with pBE2113/CvFad2 did not increase compared with that of the wild-type plant. However, an increase in the C18:3 content by 2.4% was observed in the line of No. 19 although the increase was not statistically significant. In the plant transformed with pBE2113/CvFad3, the C18:3 content increased by 6.4% compared with that of the wild-type plant. Measurement of the electrolyte leakage of the leaves showed that freezing tolerance of the CvFad2 plant was a little higher than that of the wild-type plant at all temperatures investigated (from -1 to -4°C). Contrary to the case of the CvFad2, the freezing tolerance of the CvFad3 plant tended to be slightly lower than that of the wild-type at all temperatures tested despite the unsaturation levels of the plant were higher than those of the CvFad2 plants.
|Number of pages||11|
|Journal||Journal of the Faculty of Agriculture, Kyushu University|
|Publication status||Published - Feb 1 2003|
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science