Freezing tolerance of transgenic tobacco with increased content of unsaturated fatty acid by expressing the CvFad2 or CvFad3 gene

Hideyuki Shimizu, Naoki Furuya, Koushirou Suga, Ken-ichi Honjoh, Takahisa Miyamoto, Shoji Hatano, Masayoshi Iio

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1 Citation (Scopus)

Abstract

Two fatty acid desaturases were independently expressed in tobacco plants and freezing tolerance of the transgenic plants was investigated: the two desaturases were CvFAD2, which desaturates microsomal C18:1 (oleic acid) to C18:2 (linoleic acid), and CvFAD3, which desaturates microsomal C18:2 to C18:3 (linolenic acid). Fatty acid composition analysis showed that the C18:2 contents in the plants transformed with pBE2113/CvFad2 did not increase compared with that of the wild-type plant. However, an increase in the C18:3 content by 2.4% was observed in the line of No. 19 although the increase was not statistically significant. In the plant transformed with pBE2113/CvFad3, the C18:3 content increased by 6.4% compared with that of the wild-type plant. Measurement of the electrolyte leakage of the leaves showed that freezing tolerance of the CvFad2 plant was a little higher than that of the wild-type plant at all temperatures investigated (from -1 to -4°C). Contrary to the case of the CvFad2, the freezing tolerance of the CvFad3 plant tended to be slightly lower than that of the wild-type at all temperatures tested despite the unsaturation levels of the plant were higher than those of the CvFad2 plants.

Original languageEnglish
Pages (from-to)307-317
Number of pages11
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume47
Issue number2
Publication statusPublished - Feb 1 2003

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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