From Structure-Function Analyses to Protein Engineering for Practical Applications of DNA Ligase

Maiko Tanabe, Yoshizumi Ishino, Hirokazu Nishida

Research output: Contribution to journalReview articlepeer-review

6 Citations (Scopus)

Abstract

DNA ligases are indispensable in all living cells and ubiquitous in all organs. DNA ligases are broadly utilized in molecular biology research fields, such as genetic engineering and DNA sequencing technologies. Here we review the utilization of DNA ligases in a variety of in vitro gene manipulations, developed over the past several decades. During this period, fewer protein engineering attempts for DNA ligases have been made, as compared to those for DNA polymerases. We summarize the recent progress in the elucidation of the DNA ligation mechanisms obtained from the tertiary structures solved thus far, in each step of the ligation reaction scheme. We also present some examples of engineered DNA ligases, developed from the viewpoint of their three-dimensional structures.

Original languageEnglish
Article number267570
JournalArchaea
Volume2015
DOIs
Publication statusPublished - 2015

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Physiology
  • Ecology, Evolution, Behavior and Systematics

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