Fucoidan extract enhances the anti-cancer activity of chemotherapeutic agents in MDA-MB-231 and MCF-7 breast cancer cells

Zhongyuan Zhang, Kiichiro Teruya, Toshihiro Yoshida, Hiroshi Eto, Sanetaka Shirahata

Research output: Contribution to journalArticle

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Abstract

Fucoidan, a fucose-rich polysaccharide isolated from brown alga, is currently under investigation as a new anti-cancer compound. In the present study, fucoidan extract (FE) from Cladosiphon navae-caledoniae Kylin was prepared by enzymatic digestion. We investigated whether a combination of FE with cisplatin, tamoxifen or paclitaxel had the potential to improve the therapeutic efficacy of cancer treatment. These co-treatments significantly induced cell growth inhibition, apoptosis, as well as cell cycle modifications in MDA-MB-231 and MCF-7 cells. FE enhanced apoptosis in cancer cells that responded to treatment with three chemotherapeutic drugs with downregulation of the anti-apoptotic proteins Bcl-xL and Mcl-1. The combination treatments led to an obvious decrease in the phosphorylation of ERK and Akt in MDA-MB-231 cells, but increased the phosphorylation of ERK in MCF-7 cells. In addition, we observed that combination treatments enhanced intracellular ROS levels and reduced glutathione (GSH) levels in breast cancer cells, suggesting that induction of oxidative stress was an important event in the cell death induced by the combination treatments.

Original languageEnglish
Pages (from-to)81-98
Number of pages18
JournalMarine Drugs
Volume11
Issue number1
DOIs
Publication statusPublished - Jan 1 2013

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Breast Neoplasms
MCF-7 Cells
Neoplasms
Phosphorylation
Apoptosis
Phaeophyta
Apoptosis Regulatory Proteins
Fucose
Tamoxifen
Paclitaxel
Cisplatin
Polysaccharides
Glutathione
Digestion
Cell Cycle
Oxidative Stress
Cell Death
Down-Regulation
fucoidan
Growth

All Science Journal Classification (ASJC) codes

  • Drug Discovery

Cite this

Fucoidan extract enhances the anti-cancer activity of chemotherapeutic agents in MDA-MB-231 and MCF-7 breast cancer cells. / Zhang, Zhongyuan; Teruya, Kiichiro; Yoshida, Toshihiro; Eto, Hiroshi; Shirahata, Sanetaka.

In: Marine Drugs, Vol. 11, No. 1, 01.01.2013, p. 81-98.

Research output: Contribution to journalArticle

Zhang, Zhongyuan ; Teruya, Kiichiro ; Yoshida, Toshihiro ; Eto, Hiroshi ; Shirahata, Sanetaka. / Fucoidan extract enhances the anti-cancer activity of chemotherapeutic agents in MDA-MB-231 and MCF-7 breast cancer cells. In: Marine Drugs. 2013 ; Vol. 11, No. 1. pp. 81-98.
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