Fucoidan extract induces apoptosis in MCF-7 cells via a mechanism involving the ros-dependent JNK activation and mitochondria-mediated pathways

Zhongyuan Zhang, Kiichiro Teruya, Hiroshi Eto, Sanetaka Shirahata

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Background: Fucoidan extract (FE), an enzymatically digested compound with a low molecular weight, is extracted from brown seaweed. As a natural compound with various actions, FE is attractive, especially in Asian countries, for improving the therapeutic efficacy and safety of cancer treatment. The present study was carried out to investigate the anti-tumor properties of FE in human carcinoma cells and further examine the underlying mechanisms of its activities. Methodology/Principal Finding: FE inhibits the growth of MCF-7, MDA-MB-231, HeLa, and HT1080 cells. FE-mediated apoptosis in MCF-7 cancer cells is accompanied by DNA fragmentation, nuclear condensation, and phosphatidylserine exposure. FE induces mitochondrial membrane permeabilization (MMP) through loss of mitochondrial membrane potential (ΔΨm) and regulation of the expression of Bcl-2 family members. Release of apoptosis-inducing factor (AIF) and cytochrome c precedes MMP. AIF release causes DNA fragmentation, the final stage of apoptosis, via a caspase-independent mitochondrial pathway. Additionally, FE was found to induce phosphorylation of c-Jun N-terminal kinase (JNK), p38, and extracellular signal-regulated kinase (ERK) 1/2, and apoptosis was found to be attenuated by inhibition of JNK. Furthermore, FE-mediated apoptosis was found to involve the generation of reactive oxygen species (ROS), which are responsible for the decrease of ΔΨm and phosphorylation of JNK, p38, and ERK1/2 kinases. Conclusions/Significance: These data suggest that FE activates a caspase-independent apoptotic pathway in MCF-7 cancer cells through activation of ROS-mediated MAP kinases and regulation of the Bcl-2 family protein-mediated mitochondrial pathway. They also provide evidence that FE deserves further investigation as a natural anticancer and cancer preventive agent.

Original languageEnglish
Article numbere27441
JournalPloS one
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 11 2011

Fingerprint

fucoidan
Mitochondria
JNK Mitogen-Activated Protein Kinases
MCF-7 Cells
mitogen-activated protein kinase
mitochondria
apoptosis
Chemical activation
Apoptosis
extracts
cells
Apoptosis Inducing Factor
Phosphorylation
Mitogen-Activated Protein Kinase 3
caspases
DNA fragmentation
Cells
Mitochondrial Membranes
DNA Fragmentation
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Fucoidan extract induces apoptosis in MCF-7 cells via a mechanism involving the ros-dependent JNK activation and mitochondria-mediated pathways. / Zhang, Zhongyuan; Teruya, Kiichiro; Eto, Hiroshi; Shirahata, Sanetaka.

In: PloS one, Vol. 6, No. 11, e27441, 11.11.2011.

Research output: Contribution to journalArticle

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