Fucosylated haptoglobin is a novel marker for pancreatic cancer: A detailed analysis of the oligosaccharide structure and a possible mechanism for fucosylation

Noriko Okuyama, Yoshihito Ide, Miyako Nakano, Tsutomu Nakagawa, Kanako Yamanaka, Kenta Moriwaki, Kohei Murata, Hiroaki Ohigashi, Shigekazu Yokoyama, Hidetoshi Eguchi, Osamu Ishikawa, Toshifumi Ito, Michio Kato, Akinori Kasahara, Sunao Kawano, Jianguo Gu, Naoyuki Taniguchi, Eiji Miyoshi

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

Changes in oligosaccharide structures have been reported in certain types of malignant transformations and, thus, could be used for tumor markers in certain types of cancer. In the case of pancreatic cancer cell lines, a variety of fucosylated proteins are secreted into their conditioned media. To identify fucosylated proteins in the serum of patients with pancreatic cancer, we performed western blot analyses using Aleuria Aurantica Lectin (AAL), which is specific for fucosylated structures. An ∼40 kD protein was found to be highly fucosylated in pancreatic cancer and an N-terminal analysis revealed that it was the β chain of haptoglobin. While the appearance of fucosylated haptoglobin has been reported in other diseases such as hepatocellular carcinoma, liver cirrhosis, gastric cancer and colon cancer, the incidence was significantly higher in the case of pancreatic cancer. Fucosylated haptoglobin was observed more frequently at the advanced stage of pancreatic cancer and disappeared after an operation. A mass spectrometry analysis of haptoglobin purified from the serum of patients with pancreatic cancer and the medium from a pancreatic cancer cell line, PSN-1, showed that the α 1-3/α 1-4/α 1-6 fucosylation of haptoglobin was increased in pancreatic cancer. When a hepatoma cell line, Hep3B, was cultured with the conditioned media from pancreatic cancer cells, haptoglobin secretion was dramatically increased. These findings suggest that fucosylated haptoglobin could serve as a novel marker for pancreatic cancer. Two possibilities were considered in terms of the fucosylation of haptoglobin. One is that pancreatic cancer cells, themselves, produce fucosylated haptoglobin; the other is that pancreatic cancer produces a factor, which induces the production of fucosylated haptoglobin in the liver.

Original languageEnglish
Pages (from-to)2803-2808
Number of pages6
JournalInternational Journal of Cancer
Volume118
Issue number11
DOIs
Publication statusPublished - Jun 1 2006

Fingerprint

Haptoglobins
Oligosaccharides
Pancreatic Neoplasms
Conditioned Culture Medium
Cell Line
Colonic Neoplasms
Hepatocellular Carcinoma
Tumor Biomarkers
Lectins
Liver Cirrhosis
Stomach Neoplasms
Blood Proteins
Mass Spectrometry
Proteins
Western Blotting

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Fucosylated haptoglobin is a novel marker for pancreatic cancer : A detailed analysis of the oligosaccharide structure and a possible mechanism for fucosylation. / Okuyama, Noriko; Ide, Yoshihito; Nakano, Miyako; Nakagawa, Tsutomu; Yamanaka, Kanako; Moriwaki, Kenta; Murata, Kohei; Ohigashi, Hiroaki; Yokoyama, Shigekazu; Eguchi, Hidetoshi; Ishikawa, Osamu; Ito, Toshifumi; Kato, Michio; Kasahara, Akinori; Kawano, Sunao; Gu, Jianguo; Taniguchi, Naoyuki; Miyoshi, Eiji.

In: International Journal of Cancer, Vol. 118, No. 11, 01.06.2006, p. 2803-2808.

Research output: Contribution to journalArticle

Okuyama, N, Ide, Y, Nakano, M, Nakagawa, T, Yamanaka, K, Moriwaki, K, Murata, K, Ohigashi, H, Yokoyama, S, Eguchi, H, Ishikawa, O, Ito, T, Kato, M, Kasahara, A, Kawano, S, Gu, J, Taniguchi, N & Miyoshi, E 2006, 'Fucosylated haptoglobin is a novel marker for pancreatic cancer: A detailed analysis of the oligosaccharide structure and a possible mechanism for fucosylation', International Journal of Cancer, vol. 118, no. 11, pp. 2803-2808. https://doi.org/10.1002/ijc.21728
Okuyama, Noriko ; Ide, Yoshihito ; Nakano, Miyako ; Nakagawa, Tsutomu ; Yamanaka, Kanako ; Moriwaki, Kenta ; Murata, Kohei ; Ohigashi, Hiroaki ; Yokoyama, Shigekazu ; Eguchi, Hidetoshi ; Ishikawa, Osamu ; Ito, Toshifumi ; Kato, Michio ; Kasahara, Akinori ; Kawano, Sunao ; Gu, Jianguo ; Taniguchi, Naoyuki ; Miyoshi, Eiji. / Fucosylated haptoglobin is a novel marker for pancreatic cancer : A detailed analysis of the oligosaccharide structure and a possible mechanism for fucosylation. In: International Journal of Cancer. 2006 ; Vol. 118, No. 11. pp. 2803-2808.
@article{af2667d4afd24141a7c59c5c27c53e76,
title = "Fucosylated haptoglobin is a novel marker for pancreatic cancer: A detailed analysis of the oligosaccharide structure and a possible mechanism for fucosylation",
abstract = "Changes in oligosaccharide structures have been reported in certain types of malignant transformations and, thus, could be used for tumor markers in certain types of cancer. In the case of pancreatic cancer cell lines, a variety of fucosylated proteins are secreted into their conditioned media. To identify fucosylated proteins in the serum of patients with pancreatic cancer, we performed western blot analyses using Aleuria Aurantica Lectin (AAL), which is specific for fucosylated structures. An ∼40 kD protein was found to be highly fucosylated in pancreatic cancer and an N-terminal analysis revealed that it was the β chain of haptoglobin. While the appearance of fucosylated haptoglobin has been reported in other diseases such as hepatocellular carcinoma, liver cirrhosis, gastric cancer and colon cancer, the incidence was significantly higher in the case of pancreatic cancer. Fucosylated haptoglobin was observed more frequently at the advanced stage of pancreatic cancer and disappeared after an operation. A mass spectrometry analysis of haptoglobin purified from the serum of patients with pancreatic cancer and the medium from a pancreatic cancer cell line, PSN-1, showed that the α 1-3/α 1-4/α 1-6 fucosylation of haptoglobin was increased in pancreatic cancer. When a hepatoma cell line, Hep3B, was cultured with the conditioned media from pancreatic cancer cells, haptoglobin secretion was dramatically increased. These findings suggest that fucosylated haptoglobin could serve as a novel marker for pancreatic cancer. Two possibilities were considered in terms of the fucosylation of haptoglobin. One is that pancreatic cancer cells, themselves, produce fucosylated haptoglobin; the other is that pancreatic cancer produces a factor, which induces the production of fucosylated haptoglobin in the liver.",
author = "Noriko Okuyama and Yoshihito Ide and Miyako Nakano and Tsutomu Nakagawa and Kanako Yamanaka and Kenta Moriwaki and Kohei Murata and Hiroaki Ohigashi and Shigekazu Yokoyama and Hidetoshi Eguchi and Osamu Ishikawa and Toshifumi Ito and Michio Kato and Akinori Kasahara and Sunao Kawano and Jianguo Gu and Naoyuki Taniguchi and Eiji Miyoshi",
year = "2006",
month = "6",
day = "1",
doi = "10.1002/ijc.21728",
language = "English",
volume = "118",
pages = "2803--2808",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "11",

}

TY - JOUR

T1 - Fucosylated haptoglobin is a novel marker for pancreatic cancer

T2 - A detailed analysis of the oligosaccharide structure and a possible mechanism for fucosylation

AU - Okuyama, Noriko

AU - Ide, Yoshihito

AU - Nakano, Miyako

AU - Nakagawa, Tsutomu

AU - Yamanaka, Kanako

AU - Moriwaki, Kenta

AU - Murata, Kohei

AU - Ohigashi, Hiroaki

AU - Yokoyama, Shigekazu

AU - Eguchi, Hidetoshi

AU - Ishikawa, Osamu

AU - Ito, Toshifumi

AU - Kato, Michio

AU - Kasahara, Akinori

AU - Kawano, Sunao

AU - Gu, Jianguo

AU - Taniguchi, Naoyuki

AU - Miyoshi, Eiji

PY - 2006/6/1

Y1 - 2006/6/1

N2 - Changes in oligosaccharide structures have been reported in certain types of malignant transformations and, thus, could be used for tumor markers in certain types of cancer. In the case of pancreatic cancer cell lines, a variety of fucosylated proteins are secreted into their conditioned media. To identify fucosylated proteins in the serum of patients with pancreatic cancer, we performed western blot analyses using Aleuria Aurantica Lectin (AAL), which is specific for fucosylated structures. An ∼40 kD protein was found to be highly fucosylated in pancreatic cancer and an N-terminal analysis revealed that it was the β chain of haptoglobin. While the appearance of fucosylated haptoglobin has been reported in other diseases such as hepatocellular carcinoma, liver cirrhosis, gastric cancer and colon cancer, the incidence was significantly higher in the case of pancreatic cancer. Fucosylated haptoglobin was observed more frequently at the advanced stage of pancreatic cancer and disappeared after an operation. A mass spectrometry analysis of haptoglobin purified from the serum of patients with pancreatic cancer and the medium from a pancreatic cancer cell line, PSN-1, showed that the α 1-3/α 1-4/α 1-6 fucosylation of haptoglobin was increased in pancreatic cancer. When a hepatoma cell line, Hep3B, was cultured with the conditioned media from pancreatic cancer cells, haptoglobin secretion was dramatically increased. These findings suggest that fucosylated haptoglobin could serve as a novel marker for pancreatic cancer. Two possibilities were considered in terms of the fucosylation of haptoglobin. One is that pancreatic cancer cells, themselves, produce fucosylated haptoglobin; the other is that pancreatic cancer produces a factor, which induces the production of fucosylated haptoglobin in the liver.

AB - Changes in oligosaccharide structures have been reported in certain types of malignant transformations and, thus, could be used for tumor markers in certain types of cancer. In the case of pancreatic cancer cell lines, a variety of fucosylated proteins are secreted into their conditioned media. To identify fucosylated proteins in the serum of patients with pancreatic cancer, we performed western blot analyses using Aleuria Aurantica Lectin (AAL), which is specific for fucosylated structures. An ∼40 kD protein was found to be highly fucosylated in pancreatic cancer and an N-terminal analysis revealed that it was the β chain of haptoglobin. While the appearance of fucosylated haptoglobin has been reported in other diseases such as hepatocellular carcinoma, liver cirrhosis, gastric cancer and colon cancer, the incidence was significantly higher in the case of pancreatic cancer. Fucosylated haptoglobin was observed more frequently at the advanced stage of pancreatic cancer and disappeared after an operation. A mass spectrometry analysis of haptoglobin purified from the serum of patients with pancreatic cancer and the medium from a pancreatic cancer cell line, PSN-1, showed that the α 1-3/α 1-4/α 1-6 fucosylation of haptoglobin was increased in pancreatic cancer. When a hepatoma cell line, Hep3B, was cultured with the conditioned media from pancreatic cancer cells, haptoglobin secretion was dramatically increased. These findings suggest that fucosylated haptoglobin could serve as a novel marker for pancreatic cancer. Two possibilities were considered in terms of the fucosylation of haptoglobin. One is that pancreatic cancer cells, themselves, produce fucosylated haptoglobin; the other is that pancreatic cancer produces a factor, which induces the production of fucosylated haptoglobin in the liver.

UR - http://www.scopus.com/inward/record.url?scp=33646343959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646343959&partnerID=8YFLogxK

U2 - 10.1002/ijc.21728

DO - 10.1002/ijc.21728

M3 - Article

C2 - 16385567

AN - SCOPUS:33646343959

VL - 118

SP - 2803

EP - 2808

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 11

ER -