Function of Cyp11a1 in animal models

Meng Chun Hu, Hwei Jan Hsu, Ing Cherng Guo, Bon Chu Chung

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

CYP11A1 encodes the P450scc enzyme that catalyzes the first and rate-limiting step of steroid biosynthesis. It is expressed in the adrenals and gonads under the control of pituitary peptide hormones. Transcription factor SF-1, which binds to the CYP11A1 promoter, plays an important role in the tissue-specific and hormonally regulated expression. Transgenic mouse experiments linking the CYP11A1 promoter to a reporter gene demonstrate the importance of the SF-1-binding site in directing balanced CYP11A1 gene expression. In addition, targeted mutagenesis of Cyp11a1 also reveals the importance of this gene in steroid secretion, gene regulation, and development of genitalia. Zebrafish cyp11a1 is expressed in the gonads and interrenal glands in the adults, similar to mammals. In addition, it is expressed in yolk syncytial layer during early embryogenesis. These two animal models complement each other for the understanding of gene regulatory pathways in human diseases related to steroid imbalance.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume215
Issue number1-2
DOIs
Publication statusPublished - Feb 27 2004

Fingerprint

Cholesterol Side-Chain Cleavage Enzyme
Animals
Animal Models
Genes
Steroids
Gonads
Gene expression
Interrenal Gland
Steroidogenic Factor 1
Mutagenesis
Mammals
Genitalia
Pituitary Hormones
Peptide Hormones
Biosynthesis
Zebrafish
Regulator Genes
Reporter Genes
Transgenic Mice
Embryonic Development

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Hu, M. C., Hsu, H. J., Guo, I. C., & Chung, B. C. (2004). Function of Cyp11a1 in animal models. Molecular and Cellular Endocrinology, 215(1-2), 95-100. https://doi.org/10.1016/j.mce.2003.11.024

Function of Cyp11a1 in animal models. / Hu, Meng Chun; Hsu, Hwei Jan; Guo, Ing Cherng; Chung, Bon Chu.

In: Molecular and Cellular Endocrinology, Vol. 215, No. 1-2, 27.02.2004, p. 95-100.

Research output: Contribution to journalArticle

Hu, MC, Hsu, HJ, Guo, IC & Chung, BC 2004, 'Function of Cyp11a1 in animal models', Molecular and Cellular Endocrinology, vol. 215, no. 1-2, pp. 95-100. https://doi.org/10.1016/j.mce.2003.11.024
Hu, Meng Chun ; Hsu, Hwei Jan ; Guo, Ing Cherng ; Chung, Bon Chu. / Function of Cyp11a1 in animal models. In: Molecular and Cellular Endocrinology. 2004 ; Vol. 215, No. 1-2. pp. 95-100.
@article{db4daeedd21b43cf9450c5369938cc58,
title = "Function of Cyp11a1 in animal models",
abstract = "CYP11A1 encodes the P450scc enzyme that catalyzes the first and rate-limiting step of steroid biosynthesis. It is expressed in the adrenals and gonads under the control of pituitary peptide hormones. Transcription factor SF-1, which binds to the CYP11A1 promoter, plays an important role in the tissue-specific and hormonally regulated expression. Transgenic mouse experiments linking the CYP11A1 promoter to a reporter gene demonstrate the importance of the SF-1-binding site in directing balanced CYP11A1 gene expression. In addition, targeted mutagenesis of Cyp11a1 also reveals the importance of this gene in steroid secretion, gene regulation, and development of genitalia. Zebrafish cyp11a1 is expressed in the gonads and interrenal glands in the adults, similar to mammals. In addition, it is expressed in yolk syncytial layer during early embryogenesis. These two animal models complement each other for the understanding of gene regulatory pathways in human diseases related to steroid imbalance.",
author = "Hu, {Meng Chun} and Hsu, {Hwei Jan} and Guo, {Ing Cherng} and Chung, {Bon Chu}",
year = "2004",
month = "2",
day = "27",
doi = "10.1016/j.mce.2003.11.024",
language = "English",
volume = "215",
pages = "95--100",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

TY - JOUR

T1 - Function of Cyp11a1 in animal models

AU - Hu, Meng Chun

AU - Hsu, Hwei Jan

AU - Guo, Ing Cherng

AU - Chung, Bon Chu

PY - 2004/2/27

Y1 - 2004/2/27

N2 - CYP11A1 encodes the P450scc enzyme that catalyzes the first and rate-limiting step of steroid biosynthesis. It is expressed in the adrenals and gonads under the control of pituitary peptide hormones. Transcription factor SF-1, which binds to the CYP11A1 promoter, plays an important role in the tissue-specific and hormonally regulated expression. Transgenic mouse experiments linking the CYP11A1 promoter to a reporter gene demonstrate the importance of the SF-1-binding site in directing balanced CYP11A1 gene expression. In addition, targeted mutagenesis of Cyp11a1 also reveals the importance of this gene in steroid secretion, gene regulation, and development of genitalia. Zebrafish cyp11a1 is expressed in the gonads and interrenal glands in the adults, similar to mammals. In addition, it is expressed in yolk syncytial layer during early embryogenesis. These two animal models complement each other for the understanding of gene regulatory pathways in human diseases related to steroid imbalance.

AB - CYP11A1 encodes the P450scc enzyme that catalyzes the first and rate-limiting step of steroid biosynthesis. It is expressed in the adrenals and gonads under the control of pituitary peptide hormones. Transcription factor SF-1, which binds to the CYP11A1 promoter, plays an important role in the tissue-specific and hormonally regulated expression. Transgenic mouse experiments linking the CYP11A1 promoter to a reporter gene demonstrate the importance of the SF-1-binding site in directing balanced CYP11A1 gene expression. In addition, targeted mutagenesis of Cyp11a1 also reveals the importance of this gene in steroid secretion, gene regulation, and development of genitalia. Zebrafish cyp11a1 is expressed in the gonads and interrenal glands in the adults, similar to mammals. In addition, it is expressed in yolk syncytial layer during early embryogenesis. These two animal models complement each other for the understanding of gene regulatory pathways in human diseases related to steroid imbalance.

UR - http://www.scopus.com/inward/record.url?scp=1542725958&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542725958&partnerID=8YFLogxK

U2 - 10.1016/j.mce.2003.11.024

DO - 10.1016/j.mce.2003.11.024

M3 - Article

C2 - 15026180

AN - SCOPUS:1542725958

VL - 215

SP - 95

EP - 100

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

IS - 1-2

ER -