Abstract
Objective: To search for the functional property of cord blood (CB) cells, the effects of interleukin-18 (IL-18) on interferon-γ (IFN-γ) production of T cells or natural killer (NK) cells were compared between CB and adult peripheral blood (PB). Materials and Methods: T cells, CD45RA+ T cells, and NK cells were purified from CB and adult PB mononuclear cells using magnetic beads or a cell sorter. After stimulation with or without IL-18 in the presence of IL-12 for 48 hours (NK cells) or 72 hours (T cells or CD45RA+ T cells), IFN-γ concentration was measured in each subset. Although IL-18 induced significant IFN-γ production from both CB and adult PB T cells in the presence of IL-12, the IFN-γ levels from CB T cells were lower than those from adult PB T cells. However, CD45RA+ T cells from CB and from adult PB produced similar levels of IFN-γ after stimulation with IL-18 + IL-12. On the other hand, CB NK cells exhibited higher IFN-γ production and CD69 expression than adult PB NK cells after stimulation with IL-18 + IL-12. Cytolytic activity of CB NK cells increased to a level comparable to that of adult PB NK cells after the same IL-18/IL-12 stimulation. Conclusions: These results suggest that a low response of CB T cells to IL-18 is due to a higher proportion of naive (CD45RA+) T cells in CB, which may be one of the factors responsible for the neonatal immaturity of the immune system as well as the low incidence of graft-vs-host disease in patients receiving CB stem cell transplantation. On the other hand, a high response of CB NK cells to IL-18 may contribute to the host defense during the neonatal period and antitumor effects in CB stem cell transplantation.
| Original language | English |
|---|---|
| Pages (from-to) | 1169-1176 |
| Number of pages | 8 |
| Journal | Experimental Hematology |
| Volume | 29 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Jan 1 2001 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Hematology
- Genetics
- Cell Biology
- Cancer Research
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Functional analyses of cord blood natural killer cells and T cellsa distinctive interleukin-18 response. / Nomura, Akihiko; Takada, Hidetoshi; Jin, Cheng Hao; Tanaka, Tamami; Ohga, Shouichi; Hara, Toshiro.
In: Experimental Hematology, Vol. 29, No. 10, 01.01.2001, p. 1169-1176.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional analyses of cord blood natural killer cells and T cellsa distinctive interleukin-18 response
AU - Nomura, Akihiko
AU - Takada, Hidetoshi
AU - Jin, Cheng Hao
AU - Tanaka, Tamami
AU - Ohga, Shouichi
AU - Hara, Toshiro
PY - 2001/1/1
Y1 - 2001/1/1
N2 - Objective: To search for the functional property of cord blood (CB) cells, the effects of interleukin-18 (IL-18) on interferon-γ (IFN-γ) production of T cells or natural killer (NK) cells were compared between CB and adult peripheral blood (PB). Materials and Methods: T cells, CD45RA+ T cells, and NK cells were purified from CB and adult PB mononuclear cells using magnetic beads or a cell sorter. After stimulation with or without IL-18 in the presence of IL-12 for 48 hours (NK cells) or 72 hours (T cells or CD45RA+ T cells), IFN-γ concentration was measured in each subset. Although IL-18 induced significant IFN-γ production from both CB and adult PB T cells in the presence of IL-12, the IFN-γ levels from CB T cells were lower than those from adult PB T cells. However, CD45RA+ T cells from CB and from adult PB produced similar levels of IFN-γ after stimulation with IL-18 + IL-12. On the other hand, CB NK cells exhibited higher IFN-γ production and CD69 expression than adult PB NK cells after stimulation with IL-18 + IL-12. Cytolytic activity of CB NK cells increased to a level comparable to that of adult PB NK cells after the same IL-18/IL-12 stimulation. Conclusions: These results suggest that a low response of CB T cells to IL-18 is due to a higher proportion of naive (CD45RA+) T cells in CB, which may be one of the factors responsible for the neonatal immaturity of the immune system as well as the low incidence of graft-vs-host disease in patients receiving CB stem cell transplantation. On the other hand, a high response of CB NK cells to IL-18 may contribute to the host defense during the neonatal period and antitumor effects in CB stem cell transplantation.
AB - Objective: To search for the functional property of cord blood (CB) cells, the effects of interleukin-18 (IL-18) on interferon-γ (IFN-γ) production of T cells or natural killer (NK) cells were compared between CB and adult peripheral blood (PB). Materials and Methods: T cells, CD45RA+ T cells, and NK cells were purified from CB and adult PB mononuclear cells using magnetic beads or a cell sorter. After stimulation with or without IL-18 in the presence of IL-12 for 48 hours (NK cells) or 72 hours (T cells or CD45RA+ T cells), IFN-γ concentration was measured in each subset. Although IL-18 induced significant IFN-γ production from both CB and adult PB T cells in the presence of IL-12, the IFN-γ levels from CB T cells were lower than those from adult PB T cells. However, CD45RA+ T cells from CB and from adult PB produced similar levels of IFN-γ after stimulation with IL-18 + IL-12. On the other hand, CB NK cells exhibited higher IFN-γ production and CD69 expression than adult PB NK cells after stimulation with IL-18 + IL-12. Cytolytic activity of CB NK cells increased to a level comparable to that of adult PB NK cells after the same IL-18/IL-12 stimulation. Conclusions: These results suggest that a low response of CB T cells to IL-18 is due to a higher proportion of naive (CD45RA+) T cells in CB, which may be one of the factors responsible for the neonatal immaturity of the immune system as well as the low incidence of graft-vs-host disease in patients receiving CB stem cell transplantation. On the other hand, a high response of CB NK cells to IL-18 may contribute to the host defense during the neonatal period and antitumor effects in CB stem cell transplantation.
UR - http://www.scopus.com/inward/record.url?scp=0034815728&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034815728&partnerID=8YFLogxK
U2 - 10.1016/S0301-472X(01)00689-0
DO - 10.1016/S0301-472X(01)00689-0
M3 - Article
C2 - 11602318
AN - SCOPUS:0034815728
VL - 29
SP - 1169
EP - 1176
JO - Experimental Hematology
JF - Experimental Hematology
SN - 0301-472X
IS - 10
ER -