Functional analysis by inducible RNA interference in Drosophila melanogaster.

Yuichi Matsushima, Cristina Adán, Rafael Garesse, Laurie S. Kaguni

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ribonucleic acid (RNA) interference triggered by double-stranded RNA has become a powerful tool for generating loss-of-function phenotypes. It is used to inactivate genes of interest and represents an elegant approach to genome functional analysis by reverse genetics. In Drosophila, RNA interference has been used in both cell culture and animals. We have adopted this approach to reveal the physiological roles of a number of proteins involved in mitochondrial deoxyribonucleic acid metabolism, and present here experimental schemes to induce the stable expression of double-stranded RNA in Schneider cells and in transgenic Drosophila.

Original languageEnglish
Pages (from-to)207-217
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume372
Publication statusPublished - 2007

Fingerprint

Drosophila melanogaster
RNA
Drosophila
Reverse Genetics
Cell Culture Techniques
Genome
Phenotype
DNA
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Functional analysis by inducible RNA interference in Drosophila melanogaster. / Matsushima, Yuichi; Adán, Cristina; Garesse, Rafael; Kaguni, Laurie S.

In: Methods in molecular biology (Clifton, N.J.), Vol. 372, 2007, p. 207-217.

Research output: Contribution to journalArticle

Matsushima, Yuichi ; Adán, Cristina ; Garesse, Rafael ; Kaguni, Laurie S. / Functional analysis by inducible RNA interference in Drosophila melanogaster. In: Methods in molecular biology (Clifton, N.J.). 2007 ; Vol. 372. pp. 207-217.
@article{ca9b07224c5548aab514b6f649aa8c7e,
title = "Functional analysis by inducible RNA interference in Drosophila melanogaster.",
abstract = "Ribonucleic acid (RNA) interference triggered by double-stranded RNA has become a powerful tool for generating loss-of-function phenotypes. It is used to inactivate genes of interest and represents an elegant approach to genome functional analysis by reverse genetics. In Drosophila, RNA interference has been used in both cell culture and animals. We have adopted this approach to reveal the physiological roles of a number of proteins involved in mitochondrial deoxyribonucleic acid metabolism, and present here experimental schemes to induce the stable expression of double-stranded RNA in Schneider cells and in transgenic Drosophila.",
author = "Yuichi Matsushima and Cristina Ad{\'a}n and Rafael Garesse and Kaguni, {Laurie S.}",
year = "2007",
language = "English",
volume = "372",
pages = "207--217",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Functional analysis by inducible RNA interference in Drosophila melanogaster.

AU - Matsushima, Yuichi

AU - Adán, Cristina

AU - Garesse, Rafael

AU - Kaguni, Laurie S.

PY - 2007

Y1 - 2007

N2 - Ribonucleic acid (RNA) interference triggered by double-stranded RNA has become a powerful tool for generating loss-of-function phenotypes. It is used to inactivate genes of interest and represents an elegant approach to genome functional analysis by reverse genetics. In Drosophila, RNA interference has been used in both cell culture and animals. We have adopted this approach to reveal the physiological roles of a number of proteins involved in mitochondrial deoxyribonucleic acid metabolism, and present here experimental schemes to induce the stable expression of double-stranded RNA in Schneider cells and in transgenic Drosophila.

AB - Ribonucleic acid (RNA) interference triggered by double-stranded RNA has become a powerful tool for generating loss-of-function phenotypes. It is used to inactivate genes of interest and represents an elegant approach to genome functional analysis by reverse genetics. In Drosophila, RNA interference has been used in both cell culture and animals. We have adopted this approach to reveal the physiological roles of a number of proteins involved in mitochondrial deoxyribonucleic acid metabolism, and present here experimental schemes to induce the stable expression of double-stranded RNA in Schneider cells and in transgenic Drosophila.

UR - http://www.scopus.com/inward/record.url?scp=40949086426&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40949086426&partnerID=8YFLogxK

M3 - Article

C2 - 18314728

AN - SCOPUS:40949086426

VL - 372

SP - 207

EP - 217

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -