Functional analysis of a 450-amino acid N-terminal fragment of phytochrome B in Arabidopsis

Yoshito Oka, Tomonao Matsushita, Nobuyoshi Mochizuki, Tomomi Suzuki, Satoru Tokutomi, Akira Nagatani

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Phytochrome, a major photoreceptor in plants, consists of two domains: the N-terminal photosensory domain and the C-terminal domain. Recently, the 651-amino acid photosensory domain of phytochrome B (phyB) has been shown to act as a functional photoreceptor in the nucleus. The phytochrome (PHY) domain, which is located at the C-terminal end of the photosensory domain, is required for the spectral integrity of phytochrome; however, little is known about the signal transduction activity of this domain. Here, we have established transgenic Arabidopsis thaliana lines expressing an N-terminal 450-amino acid fragment of phyB (N450) lacking the PHY domain on a phyB-deficient background. Analysis of these plants revealed that N450 can act as an active photoreceptor when attached to a short nuclear localization signal and β-glycuronidase. In vitro spectral analysis of reconstituted chromopeptides further indicated that the stability of the N450 Pfr form, an active form of phytochrome, is markedly reduced in comparison with the Pfr form of full-length phyB. Consistent with this, plants expressing N450 failed to respond to intermittent light applied at long intervals, indicating that N450 Pfr is short-lived in vivo. Taken together, our findings show that the PHY domain is dispensable for phyB signal transduction but is required for stabilizing the Pfr form of phyB.

Original languageEnglish
Pages (from-to)2104-2116
Number of pages13
JournalPlant Cell
Volume16
Issue number8
DOIs
Publication statusPublished - Aug 1 2004

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Phytochrome B
Phytochrome
Functional analysis
phytochrome
Arabidopsis
Amino Acids
amino acids
Signal transduction
Plant Photoreceptors
Signal Transduction
photoreceptors
Nuclear Localization Signals
Spectrum analysis
signal transduction
Light
nuclear localization signals

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Functional analysis of a 450-amino acid N-terminal fragment of phytochrome B in Arabidopsis. / Oka, Yoshito; Matsushita, Tomonao; Mochizuki, Nobuyoshi; Suzuki, Tomomi; Tokutomi, Satoru; Nagatani, Akira.

In: Plant Cell, Vol. 16, No. 8, 01.08.2004, p. 2104-2116.

Research output: Contribution to journalArticle

Oka, Yoshito ; Matsushita, Tomonao ; Mochizuki, Nobuyoshi ; Suzuki, Tomomi ; Tokutomi, Satoru ; Nagatani, Akira. / Functional analysis of a 450-amino acid N-terminal fragment of phytochrome B in Arabidopsis. In: Plant Cell. 2004 ; Vol. 16, No. 8. pp. 2104-2116.
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