TY - JOUR
T1 - Functional analysis of genes involved in the biosynthesis of enterocin NKR-5-3B, a novel circular bacteriocin
AU - Perez, Rodney H.
AU - Ishibashi, Naoki
AU - Inoue, Tomoko
AU - Himeno, Kohei
AU - Masuda, Yoshimitsu
AU - Sawa, Narukiko
AU - Zendo, Takeshi
AU - Wilaipun, Pongtep
AU - Leelawatcharamas, Vichien
AU - Nakayama, Jiro
AU - Sonomoto, Kenji
N1 - Funding Information:
Novozymes Japan Research Fund provided funding to Takeshi Zendo. Japan Society for the Promotion of Science (JSPS) provided funding to Takeshi Zendo under grant number 24380051.
Publisher Copyright:
© 2015, American Society for Microbiology. All Rights Reserved.
PY - 2016
Y1 - 2016
N2 - A putative biosynthetic gene cluster of the enterocin NKR-5-3B (Ent53B), a novel circular bacteriocin, was analyzed by sequencing the flanking regions around enkB, the Ent53B structural gene, using a fosmid library. A region approximately 9 kb in length was obtained, and the enkB1, enkB2, enkB3, and enkB4 genes, encoding putative biosynthetic proteins involved in the production, maturation, and secretion of Ent53B, were identified. We also determined the identity of proteins mediating self-immunity against the effects of Ent53B. Heterologous expression systems in various heterologous hosts, such as Enterococcus faecalis and Lactococcus lactis strains, were successfully established. The production and secretion of the mature Ent53B required the cooperative functions of five genes. Ent53B was produced only by those heterologous hosts that expressed protein products of the enkB, enkB1, enkB2, enkB3, and enkB4 genes. Moreover, self-immunity against the antimicrobial action of Ent53B was conferred by at least two independent mechanisms. Heterologous hosts harboring the intact enkB4 gene and/or a combination of intact enkB1 and enkB3 genes were immune to the inhibitory action of Ent53B.
AB - A putative biosynthetic gene cluster of the enterocin NKR-5-3B (Ent53B), a novel circular bacteriocin, was analyzed by sequencing the flanking regions around enkB, the Ent53B structural gene, using a fosmid library. A region approximately 9 kb in length was obtained, and the enkB1, enkB2, enkB3, and enkB4 genes, encoding putative biosynthetic proteins involved in the production, maturation, and secretion of Ent53B, were identified. We also determined the identity of proteins mediating self-immunity against the effects of Ent53B. Heterologous expression systems in various heterologous hosts, such as Enterococcus faecalis and Lactococcus lactis strains, were successfully established. The production and secretion of the mature Ent53B required the cooperative functions of five genes. Ent53B was produced only by those heterologous hosts that expressed protein products of the enkB, enkB1, enkB2, enkB3, and enkB4 genes. Moreover, self-immunity against the antimicrobial action of Ent53B was conferred by at least two independent mechanisms. Heterologous hosts harboring the intact enkB4 gene and/or a combination of intact enkB1 and enkB3 genes were immune to the inhibitory action of Ent53B.
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U2 - 10.1128/JB.00692-15
DO - 10.1128/JB.00692-15
M3 - Article
C2 - 26503847
AN - SCOPUS:84953897466
SN - 0021-9193
VL - 198
SP - 291
EP - 300
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 2
ER -