Functional Analysis of Human Hepatocytes Isolated From Chimeric Mouse Liver

N. Harimoto, H. Nakagawara, K. Shirabe, T. Yoshizumi, S. Itoh, T. Ikegami, Y. Soejima, Y. Maehara, Y. Ishida, C. Tateno, Y. Tanaka

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Abstract

Chimeric mice with humanized liver were first established by transplanting primary human hepatocytes (PHHs) isolated from a Japanese 27-year-old donor into complementary DNA-urokinase-type plasminogen activator/severe combined immunodeficiency mice. The PHHs from the Japanese donor increased more than 100-fold in the mouse liver, and human hepatocytes purified from the chimeric mouse liver (hcPHs) were successfully transplanted into second-passaged mice. These PHHs and hcPHs can produce human albumin and preserve many liver-specific enzyme genes, which are important for liver function. Interestingly, hepatitis B virus can be infected with these chimeric mice; hepatitis B viral DNA and hepatitis B surface antigen levels were detectable. In conclusion, hcPHs can be an ideal cell source for analysis of human hepatocytes.

Original languageEnglish
Pages (from-to)3858-3862
Number of pages5
JournalTransplantation Proceedings
Volume50
Issue number10
DOIs
Publication statusPublished - Dec 2018

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

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    Harimoto, N., Nakagawara, H., Shirabe, K., Yoshizumi, T., Itoh, S., Ikegami, T., Soejima, Y., Maehara, Y., Ishida, Y., Tateno, C., & Tanaka, Y. (2018). Functional Analysis of Human Hepatocytes Isolated From Chimeric Mouse Liver. Transplantation Proceedings, 50(10), 3858-3862. https://doi.org/10.1016/j.transproceed.2018.06.035