Functional analysis of protein disulfide isomerases in blood feeding, viability and oocyte development in Haemaphysalis longicornis ticks

Min Liao, Damdinsuren Boldbaatar, Haiyan Gong, Penglong Huang, Rika Umemiya, Thasaneeya Harnnoi, Jinlin Zhou, Tetsuya Tanaka, Hiroshi Suzuki, Xuenan Xuan, Kozo Fujisaki

Research output: Contribution to journalArticle

Abstract

Three protein disulfide isomerases from Haemaphysalis longicornis ticks (designated as HlPDI-1, HlPDI-2, and HlPDI-3) were previously identified. In order to further analyze their biological functions, the dsRNA of each HlPDI gene and one dsRNA combination of HlPDI-1/HlPDI-3 were separately injected into female ticks. Reduction of gene and protein expression of HlPDIs by RNA interference (RNAi) was demonstrated by real-time PCR, RT-PCR and Western blot analysis. In single dsRNA-injected groups, HlPDI-1 RNAi impacted tick blood feeding and oviposition, HlPDI-2 RNAi impacted tick viability and HlPDI-3 RNAi had no significant impact by itself. However, the injection of a combination of HlPDI-1/HlPDI-3 dsRNA had synergistic effects on tick viability. Furthermore, the midgut and cuticle were severely damaged in HlPDI-2 dsRNA-injected ticks and HlPDI-1/HlPDI-3 dsRNA-injected ticks, respectively, and disruption of HlPDI genes led to a significant reduction of disulfide bond-containing vitellogenin (Vg) expression in ticks. These results indicate that PDIs from H. longicornis are involved in blood feeding, viability and oocyte development, probably by mediating the formation of disulfide bond-containing proteins of the ticks and the formation of basement membrane and cuticle components such as extracellular matrix (ECM). This is the first report on the functional analysis of PDI family molecules as well as the interactions of PDI and other molecules in blood-feeding arthropods.

Original languageEnglish
Pages (from-to)285-95
Number of pages11
JournalInsect Biochemistry and Molecular Biology
Volume38
Issue number3
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Haemaphysalis longicornis
protein disulfide-isomerase
Protein Disulfide-Isomerases
Functional analysis
Ticks
Oocytes
ticks
oocytes
Blood
double-stranded RNA
viability
RNA
blood
RNA Interference
Disulfides
RNA interference
Arthropod Proteins
Genes
Vitellogenins
Molecules

Cite this

Functional analysis of protein disulfide isomerases in blood feeding, viability and oocyte development in Haemaphysalis longicornis ticks. / Liao, Min; Boldbaatar, Damdinsuren; Gong, Haiyan; Huang, Penglong; Umemiya, Rika; Harnnoi, Thasaneeya; Zhou, Jinlin; Tanaka, Tetsuya; Suzuki, Hiroshi; Xuan, Xuenan; Fujisaki, Kozo.

In: Insect Biochemistry and Molecular Biology, Vol. 38, No. 3, 03.2008, p. 285-95.

Research output: Contribution to journalArticle

Liao, Min ; Boldbaatar, Damdinsuren ; Gong, Haiyan ; Huang, Penglong ; Umemiya, Rika ; Harnnoi, Thasaneeya ; Zhou, Jinlin ; Tanaka, Tetsuya ; Suzuki, Hiroshi ; Xuan, Xuenan ; Fujisaki, Kozo. / Functional analysis of protein disulfide isomerases in blood feeding, viability and oocyte development in Haemaphysalis longicornis ticks. In: Insect Biochemistry and Molecular Biology. 2008 ; Vol. 38, No. 3. pp. 285-95.
@article{a227dda417fe46ce8512a79882eebe42,
title = "Functional analysis of protein disulfide isomerases in blood feeding, viability and oocyte development in Haemaphysalis longicornis ticks",
abstract = "Three protein disulfide isomerases from Haemaphysalis longicornis ticks (designated as HlPDI-1, HlPDI-2, and HlPDI-3) were previously identified. In order to further analyze their biological functions, the dsRNA of each HlPDI gene and one dsRNA combination of HlPDI-1/HlPDI-3 were separately injected into female ticks. Reduction of gene and protein expression of HlPDIs by RNA interference (RNAi) was demonstrated by real-time PCR, RT-PCR and Western blot analysis. In single dsRNA-injected groups, HlPDI-1 RNAi impacted tick blood feeding and oviposition, HlPDI-2 RNAi impacted tick viability and HlPDI-3 RNAi had no significant impact by itself. However, the injection of a combination of HlPDI-1/HlPDI-3 dsRNA had synergistic effects on tick viability. Furthermore, the midgut and cuticle were severely damaged in HlPDI-2 dsRNA-injected ticks and HlPDI-1/HlPDI-3 dsRNA-injected ticks, respectively, and disruption of HlPDI genes led to a significant reduction of disulfide bond-containing vitellogenin (Vg) expression in ticks. These results indicate that PDIs from H. longicornis are involved in blood feeding, viability and oocyte development, probably by mediating the formation of disulfide bond-containing proteins of the ticks and the formation of basement membrane and cuticle components such as extracellular matrix (ECM). This is the first report on the functional analysis of PDI family molecules as well as the interactions of PDI and other molecules in blood-feeding arthropods.",
author = "Min Liao and Damdinsuren Boldbaatar and Haiyan Gong and Penglong Huang and Rika Umemiya and Thasaneeya Harnnoi and Jinlin Zhou and Tetsuya Tanaka and Hiroshi Suzuki and Xuenan Xuan and Kozo Fujisaki",
year = "2008",
month = "3",
doi = "10.1016/j.ibmb.2007.11.006",
language = "English",
volume = "38",
pages = "285--95",
journal = "Insect Biochemistry and Molecular Biology",
issn = "0965-1748",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Functional analysis of protein disulfide isomerases in blood feeding, viability and oocyte development in Haemaphysalis longicornis ticks

AU - Liao, Min

AU - Boldbaatar, Damdinsuren

AU - Gong, Haiyan

AU - Huang, Penglong

AU - Umemiya, Rika

AU - Harnnoi, Thasaneeya

AU - Zhou, Jinlin

AU - Tanaka, Tetsuya

AU - Suzuki, Hiroshi

AU - Xuan, Xuenan

AU - Fujisaki, Kozo

PY - 2008/3

Y1 - 2008/3

N2 - Three protein disulfide isomerases from Haemaphysalis longicornis ticks (designated as HlPDI-1, HlPDI-2, and HlPDI-3) were previously identified. In order to further analyze their biological functions, the dsRNA of each HlPDI gene and one dsRNA combination of HlPDI-1/HlPDI-3 were separately injected into female ticks. Reduction of gene and protein expression of HlPDIs by RNA interference (RNAi) was demonstrated by real-time PCR, RT-PCR and Western blot analysis. In single dsRNA-injected groups, HlPDI-1 RNAi impacted tick blood feeding and oviposition, HlPDI-2 RNAi impacted tick viability and HlPDI-3 RNAi had no significant impact by itself. However, the injection of a combination of HlPDI-1/HlPDI-3 dsRNA had synergistic effects on tick viability. Furthermore, the midgut and cuticle were severely damaged in HlPDI-2 dsRNA-injected ticks and HlPDI-1/HlPDI-3 dsRNA-injected ticks, respectively, and disruption of HlPDI genes led to a significant reduction of disulfide bond-containing vitellogenin (Vg) expression in ticks. These results indicate that PDIs from H. longicornis are involved in blood feeding, viability and oocyte development, probably by mediating the formation of disulfide bond-containing proteins of the ticks and the formation of basement membrane and cuticle components such as extracellular matrix (ECM). This is the first report on the functional analysis of PDI family molecules as well as the interactions of PDI and other molecules in blood-feeding arthropods.

AB - Three protein disulfide isomerases from Haemaphysalis longicornis ticks (designated as HlPDI-1, HlPDI-2, and HlPDI-3) were previously identified. In order to further analyze their biological functions, the dsRNA of each HlPDI gene and one dsRNA combination of HlPDI-1/HlPDI-3 were separately injected into female ticks. Reduction of gene and protein expression of HlPDIs by RNA interference (RNAi) was demonstrated by real-time PCR, RT-PCR and Western blot analysis. In single dsRNA-injected groups, HlPDI-1 RNAi impacted tick blood feeding and oviposition, HlPDI-2 RNAi impacted tick viability and HlPDI-3 RNAi had no significant impact by itself. However, the injection of a combination of HlPDI-1/HlPDI-3 dsRNA had synergistic effects on tick viability. Furthermore, the midgut and cuticle were severely damaged in HlPDI-2 dsRNA-injected ticks and HlPDI-1/HlPDI-3 dsRNA-injected ticks, respectively, and disruption of HlPDI genes led to a significant reduction of disulfide bond-containing vitellogenin (Vg) expression in ticks. These results indicate that PDIs from H. longicornis are involved in blood feeding, viability and oocyte development, probably by mediating the formation of disulfide bond-containing proteins of the ticks and the formation of basement membrane and cuticle components such as extracellular matrix (ECM). This is the first report on the functional analysis of PDI family molecules as well as the interactions of PDI and other molecules in blood-feeding arthropods.

U2 - 10.1016/j.ibmb.2007.11.006

DO - 10.1016/j.ibmb.2007.11.006

M3 - Article

C2 - 18252243

VL - 38

SP - 285

EP - 295

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

SN - 0965-1748

IS - 3

ER -