Functional analysis of rat renal organic anion transporter OAT-K1: Bidirectional methotrexate transport in apical membrane

Satohiro Masuda, Ayako Takeuchi, Hideyuki Saito, Yukiya Hashimoto, Ken Ichi Inui

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Renal organic anion transporter OAT-K1 was stably transfected in MDCK cells and examined for its transport characteristics and membrane localization. OAT-K1 mediated both uptake and efflux of methotrexate in the apical membranes. Immunoblotting showed that the apparent molecular mass of the expressed OAT-K1 was 50 kDa, which was comparable to that found in the rat renal brush-border membranes. The OAT-K1-mediated methotrexate transport was significantly inhibited in the presence of several organic anions such as folate and sulfobromophthalein. These findings suggest that OAT-K1 mediates bidirectional methotrexate transport across the apical membranes, and may be involved in the renal handling of methotrexate. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)128-132
Number of pages5
JournalFEBS Letters
Volume459
Issue number1
DOIs
Publication statusPublished - Oct 1 1999

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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