Functional analysis of rat renal organic anion transporter OAT-K1: Bidirectional methotrexate transport in apical membrane

Satohiro Masuda, Ayako Takeuchi, Hideyuki Saito, Yukiya Hashimoto, Ken Ichi Inui

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Renal organic anion transporter OAT-K1 was stably transfected in MDCK cells and examined for its transport characteristics and membrane localization. OAT-K1 mediated both uptake and efflux of methotrexate in the apical membranes. Immunoblotting showed that the apparent molecular mass of the expressed OAT-K1 was 50 kDa, which was comparable to that found in the rat renal brush-border membranes. The OAT-K1-mediated methotrexate transport was significantly inhibited in the presence of several organic anions such as folate and sulfobromophthalein. These findings suggest that OAT-K1 mediates bidirectional methotrexate transport across the apical membranes, and may be involved in the renal handling of methotrexate. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)128-132
Number of pages5
JournalFEBS Letters
Volume459
Issue number1
DOIs
Publication statusPublished - Oct 1 1999
Externally publishedYes

Fingerprint

Organic Anion Transporters
Functional analysis
Methotrexate
Rats
Membranes
Kidney
Sulfobromophthalein
Madin Darby Canine Kidney Cells
Molecular mass
Brushes
Microvilli
Folic Acid
Immunoblotting
Anions

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Functional analysis of rat renal organic anion transporter OAT-K1 : Bidirectional methotrexate transport in apical membrane. / Masuda, Satohiro; Takeuchi, Ayako; Saito, Hideyuki; Hashimoto, Yukiya; Inui, Ken Ichi.

In: FEBS Letters, Vol. 459, No. 1, 01.10.1999, p. 128-132.

Research output: Contribution to journalArticle

Masuda, Satohiro ; Takeuchi, Ayako ; Saito, Hideyuki ; Hashimoto, Yukiya ; Inui, Ken Ichi. / Functional analysis of rat renal organic anion transporter OAT-K1 : Bidirectional methotrexate transport in apical membrane. In: FEBS Letters. 1999 ; Vol. 459, No. 1. pp. 128-132.
@article{4e2b43915e774f79bb499f7df6cc9cab,
title = "Functional analysis of rat renal organic anion transporter OAT-K1: Bidirectional methotrexate transport in apical membrane",
abstract = "Renal organic anion transporter OAT-K1 was stably transfected in MDCK cells and examined for its transport characteristics and membrane localization. OAT-K1 mediated both uptake and efflux of methotrexate in the apical membranes. Immunoblotting showed that the apparent molecular mass of the expressed OAT-K1 was 50 kDa, which was comparable to that found in the rat renal brush-border membranes. The OAT-K1-mediated methotrexate transport was significantly inhibited in the presence of several organic anions such as folate and sulfobromophthalein. These findings suggest that OAT-K1 mediates bidirectional methotrexate transport across the apical membranes, and may be involved in the renal handling of methotrexate. Copyright (C) 1999 Federation of European Biochemical Societies.",
author = "Satohiro Masuda and Ayako Takeuchi and Hideyuki Saito and Yukiya Hashimoto and Inui, {Ken Ichi}",
year = "1999",
month = "10",
day = "1",
doi = "10.1016/S0014-5793(99)01221-1",
language = "English",
volume = "459",
pages = "128--132",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Functional analysis of rat renal organic anion transporter OAT-K1

T2 - Bidirectional methotrexate transport in apical membrane

AU - Masuda, Satohiro

AU - Takeuchi, Ayako

AU - Saito, Hideyuki

AU - Hashimoto, Yukiya

AU - Inui, Ken Ichi

PY - 1999/10/1

Y1 - 1999/10/1

N2 - Renal organic anion transporter OAT-K1 was stably transfected in MDCK cells and examined for its transport characteristics and membrane localization. OAT-K1 mediated both uptake and efflux of methotrexate in the apical membranes. Immunoblotting showed that the apparent molecular mass of the expressed OAT-K1 was 50 kDa, which was comparable to that found in the rat renal brush-border membranes. The OAT-K1-mediated methotrexate transport was significantly inhibited in the presence of several organic anions such as folate and sulfobromophthalein. These findings suggest that OAT-K1 mediates bidirectional methotrexate transport across the apical membranes, and may be involved in the renal handling of methotrexate. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - Renal organic anion transporter OAT-K1 was stably transfected in MDCK cells and examined for its transport characteristics and membrane localization. OAT-K1 mediated both uptake and efflux of methotrexate in the apical membranes. Immunoblotting showed that the apparent molecular mass of the expressed OAT-K1 was 50 kDa, which was comparable to that found in the rat renal brush-border membranes. The OAT-K1-mediated methotrexate transport was significantly inhibited in the presence of several organic anions such as folate and sulfobromophthalein. These findings suggest that OAT-K1 mediates bidirectional methotrexate transport across the apical membranes, and may be involved in the renal handling of methotrexate. Copyright (C) 1999 Federation of European Biochemical Societies.

UR - http://www.scopus.com/inward/record.url?scp=0032859176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032859176&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(99)01221-1

DO - 10.1016/S0014-5793(99)01221-1

M3 - Article

C2 - 10508931

AN - SCOPUS:0032859176

VL - 459

SP - 128

EP - 132

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -