Functional analysis of the biosynthetic gene cluster required for immunity and secretion of a novel Lactococcus-specific bacteriocin, lactococcin Z

G. M. Daba, N. Ishibashi, Takeshi Zendo, Kenji Sonomoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aims: Characterization of the biosynthesis (secretion and immunity) of lactococcin Z. Methods and Results: Lactococcin Z is produced by Lactococcus lactisQU 7. DNA sequence analysis revealed that the lactococcin Z gene cluster (c. 5·1 kb) includes four genes encoding putative biosynthetic proteins, LczB (self-immunity protein), LczC (an ABC transporter) and LczD (a transport accessory protein), besides the previously identified LczA. LczB showed 25·5% identity to LciA, the lactococcin A immunity protein, while LczC and LczD had 93·7 and 95·3% identities, respectively, to corresponding proteins of lactococcin A. Heterologous expression of various combinations of the four genes indicated that lczB confers self-immunity against lactococcin Z, and that the four genes are necessary to produce lactococcin Z. However, LczB and LciA showed no cross-immunity to lactococcins A and Z respectively. Conclusions: The results verified that LczB is the lactococcin Z immunity protein, and LczC is responsible for lactococcin Z secretion in a manner dependent on LczD expression. Significance and Impact of the Study: The biosynthesis (secretion and immunity) of a new Lactococcus-specific bacteriocin, lactococcin Z, was characterized. Moreover, the results suggested that lactococcin Z has different immunity and action mechanisms from other Lactococcus-specific bacteriocins.

Original languageEnglish
Pages (from-to)1124-1132
Number of pages9
JournalJournal of Applied Microbiology
Volume123
Issue number5
DOIs
Publication statusPublished - Nov 1 2017

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Lactococcus
Bacteriocins
Multigene Family
Immunity
lactococcin A
Genes
Proteins
ATP-Binding Cassette Transporters
DNA Sequence Analysis
Recombinant Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Functional analysis of the biosynthetic gene cluster required for immunity and secretion of a novel Lactococcus-specific bacteriocin, lactococcin Z. / Daba, G. M.; Ishibashi, N.; Zendo, Takeshi; Sonomoto, Kenji.

In: Journal of Applied Microbiology, Vol. 123, No. 5, 01.11.2017, p. 1124-1132.

Research output: Contribution to journalArticle

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abstract = "Aims: Characterization of the biosynthesis (secretion and immunity) of lactococcin Z. Methods and Results: Lactococcin Z is produced by Lactococcus lactisQU 7. DNA sequence analysis revealed that the lactococcin Z gene cluster (c. 5·1 kb) includes four genes encoding putative biosynthetic proteins, LczB (self-immunity protein), LczC (an ABC transporter) and LczD (a transport accessory protein), besides the previously identified LczA. LczB showed 25·5{\%} identity to LciA, the lactococcin A immunity protein, while LczC and LczD had 93·7 and 95·3{\%} identities, respectively, to corresponding proteins of lactococcin A. Heterologous expression of various combinations of the four genes indicated that lczB confers self-immunity against lactococcin Z, and that the four genes are necessary to produce lactococcin Z. However, LczB and LciA showed no cross-immunity to lactococcins A and Z respectively. Conclusions: The results verified that LczB is the lactococcin Z immunity protein, and LczC is responsible for lactococcin Z secretion in a manner dependent on LczD expression. Significance and Impact of the Study: The biosynthesis (secretion and immunity) of a new Lactococcus-specific bacteriocin, lactococcin Z, was characterized. Moreover, the results suggested that lactococcin Z has different immunity and action mechanisms from other Lactococcus-specific bacteriocins.",
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N2 - Aims: Characterization of the biosynthesis (secretion and immunity) of lactococcin Z. Methods and Results: Lactococcin Z is produced by Lactococcus lactisQU 7. DNA sequence analysis revealed that the lactococcin Z gene cluster (c. 5·1 kb) includes four genes encoding putative biosynthetic proteins, LczB (self-immunity protein), LczC (an ABC transporter) and LczD (a transport accessory protein), besides the previously identified LczA. LczB showed 25·5% identity to LciA, the lactococcin A immunity protein, while LczC and LczD had 93·7 and 95·3% identities, respectively, to corresponding proteins of lactococcin A. Heterologous expression of various combinations of the four genes indicated that lczB confers self-immunity against lactococcin Z, and that the four genes are necessary to produce lactococcin Z. However, LczB and LciA showed no cross-immunity to lactococcins A and Z respectively. Conclusions: The results verified that LczB is the lactococcin Z immunity protein, and LczC is responsible for lactococcin Z secretion in a manner dependent on LczD expression. Significance and Impact of the Study: The biosynthesis (secretion and immunity) of a new Lactococcus-specific bacteriocin, lactococcin Z, was characterized. Moreover, the results suggested that lactococcin Z has different immunity and action mechanisms from other Lactococcus-specific bacteriocins.

AB - Aims: Characterization of the biosynthesis (secretion and immunity) of lactococcin Z. Methods and Results: Lactococcin Z is produced by Lactococcus lactisQU 7. DNA sequence analysis revealed that the lactococcin Z gene cluster (c. 5·1 kb) includes four genes encoding putative biosynthetic proteins, LczB (self-immunity protein), LczC (an ABC transporter) and LczD (a transport accessory protein), besides the previously identified LczA. LczB showed 25·5% identity to LciA, the lactococcin A immunity protein, while LczC and LczD had 93·7 and 95·3% identities, respectively, to corresponding proteins of lactococcin A. Heterologous expression of various combinations of the four genes indicated that lczB confers self-immunity against lactococcin Z, and that the four genes are necessary to produce lactococcin Z. However, LczB and LciA showed no cross-immunity to lactococcins A and Z respectively. Conclusions: The results verified that LczB is the lactococcin Z immunity protein, and LczC is responsible for lactococcin Z secretion in a manner dependent on LczD expression. Significance and Impact of the Study: The biosynthesis (secretion and immunity) of a new Lactococcus-specific bacteriocin, lactococcin Z, was characterized. Moreover, the results suggested that lactococcin Z has different immunity and action mechanisms from other Lactococcus-specific bacteriocins.

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