Abstract
OX40/OX40 ligand (OX40L) proteins play critical roles in the T cell-B cell and T cell-dendritic cell interactions. Here we describe the intercellular transfer of OX40L molecules by a non-Ag specific manner. After 2-h coculture of activated CD4+ T cell (OX40L-, OX40+) with FLAG peptide-tagged OX40L (OX40L-flag) protein-expressing COS-1 cells, the OX40L-flag protein was detected on the cell surface of the CD4+ T cells by both anti-OX40L and anti-FLAG mAbs. The intercellular OX40L transfer was specifically abrogated by pretreatment of the COS-1 cells with anti-OX40L mAb, 5A8. The OX40L transfer to OX40-negative cells was also observed, indicating an OX40-independent pathway of OX40L transfer. HUVECs, allostimulated monocytes, and human T cell leukemia virus type I-infected T cells, which all express OX40L, can potentially act as the donor cells of OX40L. The entire molecule of OX40L was transferred and stabilized on the recipient cell membrane with discrete punctate formation. The transferred OX40L on normal CD4+ T cells was functionally active as they stimulated latent HIV-1-infected cells to produce viral proteins via OX40 signaling. Therefore, these findings suggest that the intercellular molecular transfer of functional OX40L may be involved in modifying the immune responses.
| Original language | English |
|---|---|
| Pages (from-to) | 875-883 |
| Number of pages | 9 |
| Journal | Journal of Immunology |
| Volume | 167 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jul 15 2001 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
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Functional CD4 T cells after intercellular molecular transfer of OX40 ligand. / Baba, E.; Takahashi, Y.; Lichtenfeld, J.; Tanaka, R.; Yoshida, A.; Sugamura, K.; Yamamoto, N.; Tanaka, Y.
In: Journal of Immunology, Vol. 167, No. 2, 15.07.2001, p. 875-883.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional CD4 T cells after intercellular molecular transfer of OX40 ligand
AU - Baba, E.
AU - Takahashi, Y.
AU - Lichtenfeld, J.
AU - Tanaka, R.
AU - Yoshida, A.
AU - Sugamura, K.
AU - Yamamoto, N.
AU - Tanaka, Y.
PY - 2001/7/15
Y1 - 2001/7/15
N2 - OX40/OX40 ligand (OX40L) proteins play critical roles in the T cell-B cell and T cell-dendritic cell interactions. Here we describe the intercellular transfer of OX40L molecules by a non-Ag specific manner. After 2-h coculture of activated CD4+ T cell (OX40L-, OX40+) with FLAG peptide-tagged OX40L (OX40L-flag) protein-expressing COS-1 cells, the OX40L-flag protein was detected on the cell surface of the CD4+ T cells by both anti-OX40L and anti-FLAG mAbs. The intercellular OX40L transfer was specifically abrogated by pretreatment of the COS-1 cells with anti-OX40L mAb, 5A8. The OX40L transfer to OX40-negative cells was also observed, indicating an OX40-independent pathway of OX40L transfer. HUVECs, allostimulated monocytes, and human T cell leukemia virus type I-infected T cells, which all express OX40L, can potentially act as the donor cells of OX40L. The entire molecule of OX40L was transferred and stabilized on the recipient cell membrane with discrete punctate formation. The transferred OX40L on normal CD4+ T cells was functionally active as they stimulated latent HIV-1-infected cells to produce viral proteins via OX40 signaling. Therefore, these findings suggest that the intercellular molecular transfer of functional OX40L may be involved in modifying the immune responses.
AB - OX40/OX40 ligand (OX40L) proteins play critical roles in the T cell-B cell and T cell-dendritic cell interactions. Here we describe the intercellular transfer of OX40L molecules by a non-Ag specific manner. After 2-h coculture of activated CD4+ T cell (OX40L-, OX40+) with FLAG peptide-tagged OX40L (OX40L-flag) protein-expressing COS-1 cells, the OX40L-flag protein was detected on the cell surface of the CD4+ T cells by both anti-OX40L and anti-FLAG mAbs. The intercellular OX40L transfer was specifically abrogated by pretreatment of the COS-1 cells with anti-OX40L mAb, 5A8. The OX40L transfer to OX40-negative cells was also observed, indicating an OX40-independent pathway of OX40L transfer. HUVECs, allostimulated monocytes, and human T cell leukemia virus type I-infected T cells, which all express OX40L, can potentially act as the donor cells of OX40L. The entire molecule of OX40L was transferred and stabilized on the recipient cell membrane with discrete punctate formation. The transferred OX40L on normal CD4+ T cells was functionally active as they stimulated latent HIV-1-infected cells to produce viral proteins via OX40 signaling. Therefore, these findings suggest that the intercellular molecular transfer of functional OX40L may be involved in modifying the immune responses.
UR - http://www.scopus.com/inward/record.url?scp=0035879205&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035879205&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.167.2.875
DO - 10.4049/jimmunol.167.2.875
M3 - Article
C2 - 11441094
AN - SCOPUS:0035879205
VL - 167
SP - 875
EP - 883
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 2
ER -