Functional conservation between fission yeast moc1/sds23 and its two orthologs, budding yeast SDS23 and SDS24, and phenotypic differences in their disruptants

Md Muniruzzaman Goldar, Toshikazu Nishie, Yasue Ishikura, Toshifumi Fukuda, Kaoru Takegawa, Makoto Kawamukai

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The moc1/sds23 gene was isolated to induce sexual development of a sterile strain due to overexpression of adenylate cyclase in Schizosaccharomyces pombe. Here, we studied the functional conservation between moc1/ sds23 and its two orthologs SDS23 and SDS24 in Saccharomyces cerevisiae. We observed that the temperature sensitivity, salt tolerance, cell morphology, and sterility of the Δmoc1 mutant in S. pombe were recovered by expressing either S. cerevisiae SDS23 or SDS24. We found that deletion of both SDS23 and SDS24 resulted in the production of a large vacuole that was reversed by the expression of S. pombe moc1/sds23. In these ways we found that S. pombe Moc1/Sds23 and S. cerevisiae SDS23p or SDS24p are functional homologs. In addition we found that the Δsds23 Δsds24 diploid strain reduces cell separation in forming pseudohyphal-like growth in S. cerevisiae. Thus S. pombe moc1/sds23 and S. cerevisiae SDS23 or SDS24 are interchangeable with each other, but their disruptants are phenotypically dissimilar.

Original languageEnglish
Pages (from-to)1422-1426
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number7
DOIs
Publication statusPublished - Sep 1 2005
Externally publishedYes

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Saccharomycetales
Schizosaccharomyces
Yeast
Saccharomyces cerevisiae
Conservation
Salt-Tolerance
Sexual Development
Cell Separation
Vacuoles
Diploidy
Adenylyl Cyclases
Infertility
Salts
Genes
Temperature
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Functional conservation between fission yeast moc1/sds23 and its two orthologs, budding yeast SDS23 and SDS24, and phenotypic differences in their disruptants. / Goldar, Md Muniruzzaman; Nishie, Toshikazu; Ishikura, Yasue; Fukuda, Toshifumi; Takegawa, Kaoru; Kawamukai, Makoto.

In: Bioscience, Biotechnology and Biochemistry, Vol. 69, No. 7, 01.09.2005, p. 1422-1426.

Research output: Contribution to journalArticle

Goldar, Md Muniruzzaman ; Nishie, Toshikazu ; Ishikura, Yasue ; Fukuda, Toshifumi ; Takegawa, Kaoru ; Kawamukai, Makoto. / Functional conservation between fission yeast moc1/sds23 and its two orthologs, budding yeast SDS23 and SDS24, and phenotypic differences in their disruptants. In: Bioscience, Biotechnology and Biochemistry. 2005 ; Vol. 69, No. 7. pp. 1422-1426.
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