Abstract
There is an increase in number of γδ T cells in the peritoneal cavity after intraperitoneal (i.p.) inoculation with Escherichia coli. The E. coli- induced γδ T cells in C3H/He mice contain a large amount of Thy-1-negative population in addition to the Thy-1-positive population. We investigated the difference between the Thy-1-positive and the Thy-1-negative γδ T cells. Although it was found that only up to 4% of the γδ T cells on day 5 after infection were in cycling phase, and that the γδ T cells did not proliferate by immobilized anti-T-cell receptor (TCR) γδ monoclonal antibody stimulation, the γδ T cells proliferated in the presence of interleukin-2 (IL-2) and IL-7. The Thy-1-negative γδ T cells showed higher proliferative response compared with the Thy-1-positive γδ T cells. Furthermore, the Thy-1-negative γδ T cells showed lower IFN-γ mRNA expression than the Thy-1-positive γδ T cells. On the other hand, both the Thy-1-positive and Thy-1-negative γδ T cells predominantly expressed Vγ1, Vγ4, Vγ5, Vγ6 and Vδ1, and no difference of V region usage was detected between them. These results suggest that functions of Thy-1-positive γδ T cells differ from Thy-1-negative γδ T cells although Thy-1-positive and Thy-1-negative γδ T cells may have similar V region repertoire and, possibly, similar antigen specificity.
| Original language | English |
|---|---|
| Pages (from-to) | 261-268 |
| Number of pages | 8 |
| Journal | Immunology |
| Volume | 88 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 1996 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
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Functional difference between Thy-1-positive and Thy-1-negative γδ T cells induced by Escherichia coli infection in mice. / Takada, H.; Matsuzaki, G.; Yoshida, H.; Sumichika, H.; Nomoto, K.
In: Immunology, Vol. 88, No. 2, 01.01.1996, p. 261-268.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional difference between Thy-1-positive and Thy-1-negative γδ T cells induced by Escherichia coli infection in mice
AU - Takada, H.
AU - Matsuzaki, G.
AU - Yoshida, H.
AU - Sumichika, H.
AU - Nomoto, K.
PY - 1996/1/1
Y1 - 1996/1/1
N2 - There is an increase in number of γδ T cells in the peritoneal cavity after intraperitoneal (i.p.) inoculation with Escherichia coli. The E. coli- induced γδ T cells in C3H/He mice contain a large amount of Thy-1-negative population in addition to the Thy-1-positive population. We investigated the difference between the Thy-1-positive and the Thy-1-negative γδ T cells. Although it was found that only up to 4% of the γδ T cells on day 5 after infection were in cycling phase, and that the γδ T cells did not proliferate by immobilized anti-T-cell receptor (TCR) γδ monoclonal antibody stimulation, the γδ T cells proliferated in the presence of interleukin-2 (IL-2) and IL-7. The Thy-1-negative γδ T cells showed higher proliferative response compared with the Thy-1-positive γδ T cells. Furthermore, the Thy-1-negative γδ T cells showed lower IFN-γ mRNA expression than the Thy-1-positive γδ T cells. On the other hand, both the Thy-1-positive and Thy-1-negative γδ T cells predominantly expressed Vγ1, Vγ4, Vγ5, Vγ6 and Vδ1, and no difference of V region usage was detected between them. These results suggest that functions of Thy-1-positive γδ T cells differ from Thy-1-negative γδ T cells although Thy-1-positive and Thy-1-negative γδ T cells may have similar V region repertoire and, possibly, similar antigen specificity.
AB - There is an increase in number of γδ T cells in the peritoneal cavity after intraperitoneal (i.p.) inoculation with Escherichia coli. The E. coli- induced γδ T cells in C3H/He mice contain a large amount of Thy-1-negative population in addition to the Thy-1-positive population. We investigated the difference between the Thy-1-positive and the Thy-1-negative γδ T cells. Although it was found that only up to 4% of the γδ T cells on day 5 after infection were in cycling phase, and that the γδ T cells did not proliferate by immobilized anti-T-cell receptor (TCR) γδ monoclonal antibody stimulation, the γδ T cells proliferated in the presence of interleukin-2 (IL-2) and IL-7. The Thy-1-negative γδ T cells showed higher proliferative response compared with the Thy-1-positive γδ T cells. Furthermore, the Thy-1-negative γδ T cells showed lower IFN-γ mRNA expression than the Thy-1-positive γδ T cells. On the other hand, both the Thy-1-positive and Thy-1-negative γδ T cells predominantly expressed Vγ1, Vγ4, Vγ5, Vγ6 and Vδ1, and no difference of V region usage was detected between them. These results suggest that functions of Thy-1-positive γδ T cells differ from Thy-1-negative γδ T cells although Thy-1-positive and Thy-1-negative γδ T cells may have similar V region repertoire and, possibly, similar antigen specificity.
UR - http://www.scopus.com/inward/record.url?scp=0029891793&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029891793&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2567.1996.tb00013.x
DO - 10.1111/j.1365-2567.1996.tb00013.x
M3 - Article
C2 - 8690459
AN - SCOPUS:0029891793
VL - 88
SP - 261
EP - 268
JO - Immunology
JF - Immunology
SN - 0019-2805
IS - 2
ER -