Functional difference between Thy-1-positive and Thy-1-negative γδ T cells induced by Escherichia coli infection in mice

H. Takada, G. Matsuzaki, H. Yoshida, H. Sumichika, K. Nomoto

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Abstract

There is an increase in number of γδ T cells in the peritoneal cavity after intraperitoneal (i.p.) inoculation with Escherichia coli. The E. coli- induced γδ T cells in C3H/He mice contain a large amount of Thy-1-negative population in addition to the Thy-1-positive population. We investigated the difference between the Thy-1-positive and the Thy-1-negative γδ T cells. Although it was found that only up to 4% of the γδ T cells on day 5 after infection were in cycling phase, and that the γδ T cells did not proliferate by immobilized anti-T-cell receptor (TCR) γδ monoclonal antibody stimulation, the γδ T cells proliferated in the presence of interleukin-2 (IL-2) and IL-7. The Thy-1-negative γδ T cells showed higher proliferative response compared with the Thy-1-positive γδ T cells. Furthermore, the Thy-1-negative γδ T cells showed lower IFN-γ mRNA expression than the Thy-1-positive γδ T cells. On the other hand, both the Thy-1-positive and Thy-1-negative γδ T cells predominantly expressed Vγ1, Vγ4, Vγ5, Vγ6 and Vδ1, and no difference of V region usage was detected between them. These results suggest that functions of Thy-1-positive γδ T cells differ from Thy-1-negative γδ T cells although Thy-1-positive and Thy-1-negative γδ T cells may have similar V region repertoire and, possibly, similar antigen specificity.

Original languageEnglish
Pages (from-to)261-268
Number of pages8
JournalImmunology
Volume88
Issue number2
DOIs
Publication statusPublished - Jan 1 1996

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Escherichia coli Infections
T-Lymphocytes
Escherichia coli
Interleukin-7
Inbred C3H Mouse
Peritoneal Cavity
T-Cell Antigen Receptor
Population

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Functional difference between Thy-1-positive and Thy-1-negative γδ T cells induced by Escherichia coli infection in mice. / Takada, H.; Matsuzaki, G.; Yoshida, H.; Sumichika, H.; Nomoto, K.

In: Immunology, Vol. 88, No. 2, 01.01.1996, p. 261-268.

Research output: Contribution to journalArticle

Takada, H. ; Matsuzaki, G. ; Yoshida, H. ; Sumichika, H. ; Nomoto, K. / Functional difference between Thy-1-positive and Thy-1-negative γδ T cells induced by Escherichia coli infection in mice. In: Immunology. 1996 ; Vol. 88, No. 2. pp. 261-268.
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