Functional domains of Rts1 and P1 RepA proteins for initiation of replication

Yong Fang Li, Tetsuya Hayashi, Yoshiro Terawaki

Research output: Contribution to journalArticle

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Abstract

Rts1 RepA and P1 RepA are trans-acting proteins essential for initiation of replication of Rts1 and P1 plasmids, respectively. We recently found that P1 RepA bound in vitro to the Rts1 replication origin as strongly as Rts1 RepA and activated the origin in vivo. However, the ori activation was quite inefficient. This study shows that by replacing a small region of P1 RepA with the corresponding region of Rts1 RepA, the efficiency of Rts1 ori activation increased markedly. Interestingly, the same subregion of P1 RepA was found to be important for in vivo activation of the P1 origin. Thus, a region essential for efficient activation of the replication origin was assigned to the P1 RepA molecule as well as to the Rts1 RepA molecule. The region was distinct from a domain necessary for in vitro binding to the origin, although both regions were required for in vivo activation of the respective origin.

Original languageEnglish
Pages (from-to)140-149
Number of pages10
JournalPlasmid
Volume40
Issue number2
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

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Replication Origin
Proteins
Plasmids
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Molecular Biology

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Functional domains of Rts1 and P1 RepA proteins for initiation of replication. / Li, Yong Fang; Hayashi, Tetsuya; Terawaki, Yoshiro.

In: Plasmid, Vol. 40, No. 2, 01.01.1998, p. 140-149.

Research output: Contribution to journalArticle

Li, Yong Fang ; Hayashi, Tetsuya ; Terawaki, Yoshiro. / Functional domains of Rts1 and P1 RepA proteins for initiation of replication. In: Plasmid. 1998 ; Vol. 40, No. 2. pp. 140-149.
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