Functional expression of Fas antigen (CD95) on hematopoietic progenitor cells

K. Nagafuji, T. Shibuya, M. Harada, S. I. Mizuno, K. Takenaka, T. Miyamoto, T. Okamura, H. Gondo, Y. Niho

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Abstract

We investigated the expression of an apoptosis-associated antigen (Fas) (CD95) on hematopoietic progenitor cells in the presence or absence of interferon-γ (IFN-γ) and/or tumor necrosis factor-α (TNF-α). CD34+ cells freshly isolated from bone marrow did not express Fas. However, IFN-γ and/or TNF-α induced the expression of both the mRNA of Fas and Fas itself in a dose-dependent fashion on the surface of CD34+ cells after 48 hours of serum-free culture. IFN-γ and TNF-α had a synergistic effect on the induction of Fas, when both cytokines were added to the culture. The TNF-α- induced Fas expression is mediated by p55 TNF-α receptor. CD34+ cells cultured in medium alone or with stem cell factor (SCF) showed some slight expression of Fas. When anti-Fas antibody (IgM) was added to CD34+ cells after the induction of Fas expression, CD34+ cells underwent apoptosis, as shown by a decrease in the number of viable cells, morphologic changes, the induction of DNA fragmentation, and a decrease in the number of colony- forming cells (CFC) including colony-forming unit granulocytes/macrophages (CFU-GM) and burst-forming unit erythroids (BFU-E). These observations indicate that IFN-γ and/or TNF-α, well known as negative hematopoietic regulators, induce functional Fas on hematopoietic progenitor cells. The suppression of hematopoiesis by negative hematopoietic regulators may be mediated in part by Fas induction.

Original languageEnglish
Pages (from-to)883-889
Number of pages7
JournalBlood
Volume86
Issue number3
Publication statusPublished - Jan 1 1995

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Nagafuji, K., Shibuya, T., Harada, M., Mizuno, S. I., Takenaka, K., Miyamoto, T., ... Niho, Y. (1995). Functional expression of Fas antigen (CD95) on hematopoietic progenitor cells. Blood, 86(3), 883-889.