By using B cells of X-linked severe combined immunodeficiency (XSCID) patients, we indicated that mutation of the IL-2 receptor y chain (y c) caused abnormal signal transduction not only of IL-2 but also of IL-4. In this study, we evaluated a functional significance of y c in IL-4 receptor system of monocytes/macrophages. Mouse monocytic cells never responded to human IL-4. We introduced human IL-4 receptor a chain (IL-4Rcc ) gene into a mouse monocytic cell line and established cell lines stably expressing human IL-4Ra. Human IL-4, as well as mouse IL-4, reduced productions of TNF-a, prostaglandins and nitric oxide inducibly produced by such transformed cell lines stimulated with lipopolysaccharide. Neutralizing antibody against y c hardly blocked these functions of human IL-4, indicating that y c may be less important in transducing signals carried out by human IL-4. Further studies are still needed to explain significance of IL-4Rcx and y c of functional IL-4 receptor.
|Number of pages||1|
|Journal||Japanese Journal of Human Genetics|
|Publication status||Published - 1997|
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