Functional expression of human interleukin-4 receptor α gene on a mouse macrophage cell line

Takeshi Qtsukar; Xoshiaki Nemoto; Kunihiro Yamaqka; Hitoshi Nakasfflma; Hiroaki Niiro; Yosuke Tanaka; Eiichi Qhgaml; Yojiro Arinqbu; Yoshiyuki Niho; Izuhara Kenji

Functional expression of human interleukin-4 receptor α gene on a mouse macrophage cell line

Takeshi Qtsukar, Xoshiaki Nemoto, Kunihiro Yamaqka, Hitoshi Nakasfflma, Hiroaki Niiro, Yosuke Tanaka, Eiichi Qhgaml, Yojiro Arinqbu, Yoshiyuki Niho, Izuhara Kenji

Medical Education

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Abstract

By using B cells of X-linked severe combined immunodeficiency (XSCID) patients, we indicated that mutation of the IL-2 receptor y chain (y c) caused abnormal signal transduction not only of IL-2 but also of IL-4. In this study, we evaluated a functional significance of y c in IL-4 receptor system of monocytes/macrophages. Mouse monocytic cells never responded to human IL-4. We introduced human IL-4 receptor a chain (IL-4Rcc ) gene into a mouse monocytic cell line and established cell lines stably expressing human IL-4Ra. Human IL-4, as well as mouse IL-4, reduced productions of TNF-a, prostaglandins and nitric oxide inducibly produced by such transformed cell lines stimulated with lipopolysaccharide. Neutralizing antibody against y c hardly blocked these functions of human IL-4, indicating that y c may be less important in transducing signals carried out by human IL-4. Further studies are still needed to explain significance of IL-4Rcx and y c of functional IL-4 receptor.

Original language	English
Pages (from-to)	74
Number of pages	1
Journal	Japanese Journal of Human Genetics
Volume	42
Issue number	1
Publication status	Published - 1997

All Science Journal Classification (ASJC) codes

Genetics(clinical)

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Functional expression of human interleukin-4 receptor α gene on a mouse macrophage cell line. / Qtsukar, Takeshi; Nemoto, Xoshiaki; Yamaqka, Kunihiro; Nakasfflma, Hitoshi; Niiro, Hiroaki; Tanaka, Yosuke; Qhgaml, Eiichi; Arinqbu, Yojiro; Niho, Yoshiyuki; Kenji, Izuhara.

In: Japanese Journal of Human Genetics, Vol. 42, No. 1, 1997, p. 74.

Research output: Contribution to journal › Article › peer-review

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abstract = "By using B cells of X-linked severe combined immunodeficiency (XSCID) patients, we indicated that mutation of the IL-2 receptor y chain (y c) caused abnormal signal transduction not only of IL-2 but also of IL-4. In this study, we evaluated a functional significance of y c in IL-4 receptor system of monocytes/macrophages. Mouse monocytic cells never responded to human IL-4. We introduced human IL-4 receptor a chain (IL-4Rcc ) gene into a mouse monocytic cell line and established cell lines stably expressing human IL-4Ra. Human IL-4, as well as mouse IL-4, reduced productions of TNF-a, prostaglandins and nitric oxide inducibly produced by such transformed cell lines stimulated with lipopolysaccharide. Neutralizing antibody against y c hardly blocked these functions of human IL-4, indicating that y c may be less important in transducing signals carried out by human IL-4. Further studies are still needed to explain significance of IL-4Rcx and y c of functional IL-4 receptor.",

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AU - Niiro, Hiroaki

AU - Tanaka, Yosuke

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AU - Niho, Yoshiyuki

AU - Kenji, Izuhara

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AB - By using B cells of X-linked severe combined immunodeficiency (XSCID) patients, we indicated that mutation of the IL-2 receptor y chain (y c) caused abnormal signal transduction not only of IL-2 but also of IL-4. In this study, we evaluated a functional significance of y c in IL-4 receptor system of monocytes/macrophages. Mouse monocytic cells never responded to human IL-4. We introduced human IL-4 receptor a chain (IL-4Rcc ) gene into a mouse monocytic cell line and established cell lines stably expressing human IL-4Ra. Human IL-4, as well as mouse IL-4, reduced productions of TNF-a, prostaglandins and nitric oxide inducibly produced by such transformed cell lines stimulated with lipopolysaccharide. Neutralizing antibody against y c hardly blocked these functions of human IL-4, indicating that y c may be less important in transducing signals carried out by human IL-4. Further studies are still needed to explain significance of IL-4Rcx and y c of functional IL-4 receptor.

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Functional expression of human interleukin-4 receptor α gene on a mouse macrophage cell line

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