Functional expression of human interleukin-4 receptor α gene on a mouse macrophage cell line

Takeshi Qtsukar, Xoshiaki Nemoto, Kunihiro Yamaqka, Hitoshi Nakasfflma, Hiroaki Niiro, Yosuke Tanaka, Eiichi Qhgaml, Yojiro Arinqbu, Yoshiyuki Niho, Izuhara Kenji

Research output: Contribution to journalArticlepeer-review

Abstract

By using B cells of X-linked severe combined immunodeficiency (XSCID) patients, we indicated that mutation of the IL-2 receptor y chain (y c) caused abnormal signal transduction not only of IL-2 but also of IL-4. In this study, we evaluated a functional significance of y c in IL-4 receptor system of monocytes/macrophages. Mouse monocytic cells never responded to human IL-4. We introduced human IL-4 receptor a chain (IL-4Rcc ) gene into a mouse monocytic cell line and established cell lines stably expressing human IL-4Ra. Human IL-4, as well as mouse IL-4, reduced productions of TNF-a, prostaglandins and nitric oxide inducibly produced by such transformed cell lines stimulated with lipopolysaccharide. Neutralizing antibody against y c hardly blocked these functions of human IL-4, indicating that y c may be less important in transducing signals carried out by human IL-4. Further studies are still needed to explain significance of IL-4Rcx and y c of functional IL-4 receptor.

Original languageEnglish
Pages (from-to)74
Number of pages1
JournalJapanese Journal of Human Genetics
Volume42
Issue number1
Publication statusPublished - 1997

All Science Journal Classification (ASJC) codes

  • Genetics(clinical)

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