Functional expression of schizosaccharomyces pombe Vba2p in the vacuolar membrane of Saccharomyces cerevisiae

Pongsanat Pongcharoen, Miyuki Kawano-Kawada, Tomoko Iwaki, Naoko Sugimoto, Takayuki Sekito, Koichi Akiyama, Kaoru Takegawa, Yoshimi Kakinuma

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A vacuolar membrane protein, Vba2p of Schizosaccharomyces pombe, is involved in basic amino acid uptake by intact cells. Here we found evidence that Vba2p mediated ATP-dependent lysine uptake by vacuolar membrane vesicles of Saccharomyces cerevisiae. Vba2p was also responsible for quinidine sensitivity, and the addition of lysine improved cell growth on quinidine-containing media. These findings should be useful for further characterization of Vba2p.

Original languageEnglish
Pages (from-to)1988-1990
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number9
DOIs
Publication statusPublished - 2013

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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