Functional implication of archaeal homologues of human RNase P protein pair Pop5 and Rpp30

Masato Hamasaki, Kohsuke Hazeyama, Fumihiko Iwasaki, Toshifumi Ueda, Takashi Nakashima, Yoshimitsu Kakuta, Makoto Kimura

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

PhoPop5 and PhoRpp30 in the hyperthermophilic archaeon Pyrococcus horikoshii, homologues of human ribonuclease P (RNase P) proteins hPop5 and Rpp30, respectively, fold into a heterotetramer [PhoRpp30-(PhoPop5)2-PhoRpp30], which plays a crucial role in the activation of RNase P RNA (PhopRNA). Here, we examined the functional implication of PhoPop5 and PhoRpp30 in the tetramer. Surface plasmon resonance (SPR) analysis revealed that the tetramer strongly interacts with an oligonucleotide including the nucleotide sequence of a stem-loop SL3 in PhopRNA. In contrast, PhoPop5 had markedly reduced affinity to SL3, whereas PhoRpp30 had little affinity to SL3. SPR studies of PhoPop5 mutants further revealed that the C-terminal helix (α4) in PhoPop5 functions as a molecular recognition element for SL3. Moreover, gel filtration indicated that PhoRpp30 exists as a monomer, whereas PhoPop5 is an oligomer in solution, suggesting that PhoRpp30 assists PhoPop5 in attaining a functionally active conformation by shielding hydrophobic surfaces of PhoPop5. These results, together with available data, allow us to generate a structural and mechanistic model for the PhopRNA activation by PhoPop5 and PhoRpp30, in which the two C-terminal helices (α4) of PhoPop5 in the tetramer whose formation is assisted by PhoRpp30 act as binding elements and bridge SL3 and SL16 in PhopRNA.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalJournal of biochemistry
Volume159
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

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Ribonuclease P
Surface Plasmon Resonance
Surface plasmon resonance
Pyrococcus horikoshii
Chemical activation
Molecular recognition
Structural Models
Archaea
Oligomers
Oligonucleotides
Shielding
Gel Chromatography
Conformations
Proteins
Nucleotides
Monomers
Gels
RNA
human OCA2 protein

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Biochemistry
  • Molecular Biology

Cite this

Functional implication of archaeal homologues of human RNase P protein pair Pop5 and Rpp30. / Hamasaki, Masato; Hazeyama, Kohsuke; Iwasaki, Fumihiko; Ueda, Toshifumi; Nakashima, Takashi; Kakuta, Yoshimitsu; Kimura, Makoto.

In: Journal of biochemistry, Vol. 159, No. 1, 01.01.2016, p. 31-40.

Research output: Contribution to journalArticle

Hamasaki, Masato ; Hazeyama, Kohsuke ; Iwasaki, Fumihiko ; Ueda, Toshifumi ; Nakashima, Takashi ; Kakuta, Yoshimitsu ; Kimura, Makoto. / Functional implication of archaeal homologues of human RNase P protein pair Pop5 and Rpp30. In: Journal of biochemistry. 2016 ; Vol. 159, No. 1. pp. 31-40.
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