Functional implications of Ca2+ mobilizing properties for nitric oxide production in aortic endothelium

Tetsuya Koyama, Chiwaka Kimura, Sung Jin Park, Masahiro Oike, Yushi Ito

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We have investigated the relationship between Ca2+ mobilization and the cellular production of nitric oxide (NO) by using fura-2 and diaminofluorescein-2 (DAF-2), an NO-sensitive dye, in bovine aortic endothelial cells (BAEC). High concentrations of ATP (100 μM) or thapsigargin (1 μM) depleted intracellular Ca2+ store sites with a single Ca2+ transient, and induced an increase in DAF-2 fluorescence even in Ca2+-free solution, thereby indicating that store depletion leads to NO production. The same level of increase in DAF-2 fluorescence was elicited by low concentrations of ATP (1 μM), which induced Ca2+ oscillations but did not deplete store sites, only in the presence of extracellular Ca2+. Furthermore, inhibition of ATP (1 μM)-induced Ca2+ entry with La3+ suppressed DAF-2 fluorescence. ATP (0.3 μM), applied in Ca2+-free, Mn2+-containing solution induced Mn2+ entry-coupled fura-2 quenching, repeating shortly after each oscillation peak. These results indicate that NO is produced preferentially by entered Ca2+, and that Ca2+ oscillations, which are induced by low levels of stimulation, play a significant role in NO production by strongly modulating Ca2+ entry.

Original languageEnglish
Pages (from-to)511-520
Number of pages10
JournalLife Sciences
Volume72
Issue number4-5
DOIs
Publication statusPublished - Dec 20 2002

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Endothelium
Nitric Oxide
Adenosine Triphosphate
Fura-2
Fluorescence
Thapsigargin
Endothelial cells
Quenching
Coloring Agents
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Functional implications of Ca2+ mobilizing properties for nitric oxide production in aortic endothelium. / Koyama, Tetsuya; Kimura, Chiwaka; Park, Sung Jin; Oike, Masahiro; Ito, Yushi.

In: Life Sciences, Vol. 72, No. 4-5, 20.12.2002, p. 511-520.

Research output: Contribution to journalArticle

Koyama, Tetsuya ; Kimura, Chiwaka ; Park, Sung Jin ; Oike, Masahiro ; Ito, Yushi. / Functional implications of Ca2+ mobilizing properties for nitric oxide production in aortic endothelium. In: Life Sciences. 2002 ; Vol. 72, No. 4-5. pp. 511-520.
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