Functional modules and expression of mouse p40(phox) and p67(phox), SH3- domain-containing proteins involved in the phagocyte NADPH oxidase complex

Kazuhito Mizuki, Kenji Kadomatsu, Kenichiro Hata, Takashi Ito, Qi Wen Fan, Yohko Kage, Yasuyuki Fukumaki, Yoshiyuki Sakaki, Koichiro Takeshige, Hideki Sumimoto

Research output: Contribution to journalArticle

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Abstract

The phagocyte NADPH oxidase is activated during phagocytosis to produce superoxide, a precursor of microbicidal oxidants. The formation of the active oxidase complex at the membrane requires translocation of the Rac GTPase and two specialized cytosolic proteins that harbor SH3 domains, p67(phox) and p47(phox). Another SH3-domain-containing protein p40(phox), which is constitutively associated with p67(phox) in phagocytes, also enters the complex upon cell stimulation. Here we describe how we cloned mouse cDNAs encoding p40(phox) and its partner in phagocytes, p67(phox). Both p40(phox) and p67(phox) comprise several protein-binding modules that are structurally and functionally well conserved between mouse and human, indicating their nature as adaptor proteins. We have also systematically investigated expression of the gene for p40(phox) in comparison with those for p67(phox) and p47(phox). Distributions of the mRNAs for the three proteins among tissues are similar, with the most abundant expression in the spleen. The messages are abundant not only in phagocytic cells, but also in B cell lineage. The p40(phox) gene, but not the other two, is expressed in some types of cells such as plasma cells and T lymphocytes. Furthermore, in situ hybridization analysis shows that the p40(phox) mRNA is distributed in neuronal cells of mouse brain, providing evidence that one of the genes for the specialized oxidase factors is expressed in neurons. These observations raise the possibility that the adaptor protein p40(phox) plays a heretofore unsuspected role via interacting with other proteins in the cells that do not express p67(phox) or p47(phox).

Original languageEnglish
Pages (from-to)573-582
Number of pages10
JournalEuropean Journal of Biochemistry
Volume251
Issue number3
DOIs
Publication statusPublished - Feb 1 1998

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src Homology Domains
NADPH Oxidase
Phagocytes
Proteins
Genes
Oxidoreductases
Cells
Messenger RNA
T-cells
GTP Phosphohydrolases
Cell Lineage
Ports and harbors
Plasma Cells
neutrophil cytosol factor 40K
neutrophil cytosol factor 67K
Phagocytosis
Protein Binding
Oxidants
Superoxides
Neurons

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Functional modules and expression of mouse p40(phox) and p67(phox), SH3- domain-containing proteins involved in the phagocyte NADPH oxidase complex. / Mizuki, Kazuhito; Kadomatsu, Kenji; Hata, Kenichiro; Ito, Takashi; Fan, Qi Wen; Kage, Yohko; Fukumaki, Yasuyuki; Sakaki, Yoshiyuki; Takeshige, Koichiro; Sumimoto, Hideki.

In: European Journal of Biochemistry, Vol. 251, No. 3, 01.02.1998, p. 573-582.

Research output: Contribution to journalArticle

Mizuki, Kazuhito ; Kadomatsu, Kenji ; Hata, Kenichiro ; Ito, Takashi ; Fan, Qi Wen ; Kage, Yohko ; Fukumaki, Yasuyuki ; Sakaki, Yoshiyuki ; Takeshige, Koichiro ; Sumimoto, Hideki. / Functional modules and expression of mouse p40(phox) and p67(phox), SH3- domain-containing proteins involved in the phagocyte NADPH oxidase complex. In: European Journal of Biochemistry. 1998 ; Vol. 251, No. 3. pp. 573-582.
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AU - Hata, Kenichiro

AU - Ito, Takashi

AU - Fan, Qi Wen

AU - Kage, Yohko

AU - Fukumaki, Yasuyuki

AU - Sakaki, Yoshiyuki

AU - Takeshige, Koichiro

AU - Sumimoto, Hideki

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