Functional properties of neurotransmitter receptors transplanted into Xenopus oocytes

H. Sugiyama, C. Hirono, I. Ito, S. Yamagishi

Research output: Contribution to journalArticle

Abstract

When oocytes of African frog Xenopus laevis were injected with mRNA isolated from rat brains, the oocyted synthesized functional properties such as ionic channels or neurotransmitter receptors based on the injected mRNA. Functional properties of neurotransmitter receptors thus transplanted from rat brains were studied in the oocytes. Analyses of intracellular reaction mechanisms of these receptors revealed that, in this system, receptors that activate G-proteins and stimulate inositol phospholipid metabolism and intracellular Ca2+ mobilization were expressed very efficiently. Muscarinic acetylcholine receptor is a typical one of this category. Neurotensin receptor was also found to belong to this category. In addition, a new subtype pf glutamate receptor was found that stimulates the same intracellular reactions. This is the first direct evidence for a glutamate receptor subtype that stimulates G-protein-mediated reactions.

Original languageEnglish
Pages (from-to)55-58
Number of pages4
JournalBiomedical Research
Volume8
Issue numberSUPPL. 1987
Publication statusPublished - Jan 1 1987

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Neurotransmitter Receptor
Glutamate Receptors
Xenopus
GTP-Binding Proteins
Oocytes
Rats
Brain
Neurotensin Receptors
Messenger RNA
Xenopus laevis
Muscarinic Receptors
Phosphatidylinositols
Ion Channels
Metabolism
Anura

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Sugiyama, H., Hirono, C., Ito, I., & Yamagishi, S. (1987). Functional properties of neurotransmitter receptors transplanted into Xenopus oocytes. Biomedical Research, 8(SUPPL. 1987), 55-58.

Functional properties of neurotransmitter receptors transplanted into Xenopus oocytes. / Sugiyama, H.; Hirono, C.; Ito, I.; Yamagishi, S.

In: Biomedical Research, Vol. 8, No. SUPPL. 1987, 01.01.1987, p. 55-58.

Research output: Contribution to journalArticle

Sugiyama, H, Hirono, C, Ito, I & Yamagishi, S 1987, 'Functional properties of neurotransmitter receptors transplanted into Xenopus oocytes', Biomedical Research, vol. 8, no. SUPPL. 1987, pp. 55-58.
Sugiyama H, Hirono C, Ito I, Yamagishi S. Functional properties of neurotransmitter receptors transplanted into Xenopus oocytes. Biomedical Research. 1987 Jan 1;8(SUPPL. 1987):55-58.
Sugiyama, H. ; Hirono, C. ; Ito, I. ; Yamagishi, S. / Functional properties of neurotransmitter receptors transplanted into Xenopus oocytes. In: Biomedical Research. 1987 ; Vol. 8, No. SUPPL. 1987. pp. 55-58.
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