Functional role of PKC in contraction of cultured human prostatic stromal cells

Ryosuke Takahashi, Junji Nishimura, Katsuya Hirano, Seiji Naito, Hideo Kanaide

Research output: Contribution to journalArticle

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Abstract

The contractile activity of prostatic stromal cells contributes to symptoms of benign prostatic hyperplasia (BPH). However, the mechanisms for this contraction have not yet been fully elucidated. In this study, we investigated the role of protein kinase C (PKC) in prostatic contraction by measuring the isometric tension development of cultured human prostatic stromal cells (CHPSCs) derived from BPH patients. Fresh human BPH tissue was used only in a Western blot analysis. A ring preparation made of CHPSCs and collagen gel could develop an isometric tension during activation with various agonists. Phorbol 12, 13 dibutyrate (PDBu), a PKC activator, induced a relaxation. A Western blot analysis revealed the expression of PKC-potentiated protein phosphatase-1 inhibitory protein (CPI-17) in both CHPSCs and fresh human BPH tissue to be much lower than that in the rabbit aorta. When CPI-17 was over-expressed, PDBu induced a large contraction, but the agonist-induced contraction did not become larger than expected. In α-toxin permeabilized preparations, PDBu induced a relaxation in control CHPSCs, while it induced a contraction at a constant [Ca2+]i in CPI-17 over-expressing CHPSCs. These results indicated that the activation of PKC in CHPSCs induces a relaxation probably due to low expression level of CPI-17 and also that the PKC-CPI-17 pathway does not appear to play a major role in the agonist-induced contraction even when CPI-17 was over-expressed.

Original languageEnglish
Pages (from-to)65-78
Number of pages14
JournalJournal of Cellular Biochemistry
Volume96
Issue number1
DOIs
Publication statusPublished - Sep 1 2005

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Stromal Cells
Protein Kinase C
Phorbol 12,13-Dibutyrate
Prostatic Hyperplasia
Chemical activation
Tissue
Protein Phosphatase 1
Western Blotting
Isometric Contraction
Human Development
cyclopropapyrroloindole
Collagen
Gels
Aorta
Cells
Rabbits
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Functional role of PKC in contraction of cultured human prostatic stromal cells. / Takahashi, Ryosuke; Nishimura, Junji; Hirano, Katsuya; Naito, Seiji; Kanaide, Hideo.

In: Journal of Cellular Biochemistry, Vol. 96, No. 1, 01.09.2005, p. 65-78.

Research output: Contribution to journalArticle

Takahashi, R, Nishimura, J, Hirano, K, Naito, S & Kanaide, H 2005, 'Functional role of PKC in contraction of cultured human prostatic stromal cells', Journal of Cellular Biochemistry, vol. 96, no. 1, pp. 65-78. https://doi.org/10.1002/jcb.20534
Takahashi, Ryosuke ; Nishimura, Junji ; Hirano, Katsuya ; Naito, Seiji ; Kanaide, Hideo. / Functional role of PKC in contraction of cultured human prostatic stromal cells. In: Journal of Cellular Biochemistry. 2005 ; Vol. 96, No. 1. pp. 65-78.
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