Functional significance of the E loop, a novel motif conserved in the lantibiotic immunity ATP-binding cassette transport systems

Ken Ichi Okuda, Sae Yanagihara, Tomomichi Sugayama, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Lantibiotics are peptide-derived antibacterial substances produced by some Gram-positive bacteria and characterized by the presence of unusual amino acids, like lanthionines and dehydrated amino acids. Because lantibiotic producers may be attacked by self-produced lantibiotics, they express immunity proteins on the cytoplasmic membrane. An ATP-binding cassette (ABC) transport system mediated by the LanFEG protein complex is a major system in lantibiotic immunity. Multiple-sequence alignment analysis revealed that LanF proteins contain the E loop, a variant of the Q loop, which is a well-conserved motif in the nucleotide-binding domains (NBDs) of general ABC transporters. To elucidate E loop function, we introduced a mutation in the NukF protein, which is involved in the nukacin-ISK-1 immunity system. Amino acid replacement of glutamic acid in the E loop with glutamine (E85Q) resulted in slight decreases in the immunity level and transport activity. Additionally, the E85A mutation severely impaired the immunity level and transport activity. On the other hand, ATPase activities of purified E85Q and E85A mutants were almost similar to that of the wild type. These results suggested that the E loop found in ABC transporters involved in lantibiotic immunity plays a significant role in the function of these transporters, especially in the structural change of transmembrane domains.

Original languageEnglish
Pages (from-to)2801-2808
Number of pages8
JournalJournal of bacteriology
Volume192
Issue number11
DOIs
Publication statusPublished - Jun 1 2010

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Bacteriocins
Immunity
Adenosine Triphosphate
ATP-Binding Cassette Transporters
Amino Acids
Proteins
Mutation
Nucleotide Motifs
Sequence Alignment
Gram-Positive Bacteria
Glutamine
Sequence Analysis
Adenosine Triphosphatases
Glutamic Acid
Cell Membrane
Peptides

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Functional significance of the E loop, a novel motif conserved in the lantibiotic immunity ATP-binding cassette transport systems. / Okuda, Ken Ichi; Yanagihara, Sae; Sugayama, Tomomichi; Zendo, Takeshi; Nakayama, Jiro; Sonomoto, Kenji.

In: Journal of bacteriology, Vol. 192, No. 11, 01.06.2010, p. 2801-2808.

Research output: Contribution to journalArticle

Okuda, Ken Ichi ; Yanagihara, Sae ; Sugayama, Tomomichi ; Zendo, Takeshi ; Nakayama, Jiro ; Sonomoto, Kenji. / Functional significance of the E loop, a novel motif conserved in the lantibiotic immunity ATP-binding cassette transport systems. In: Journal of bacteriology. 2010 ; Vol. 192, No. 11. pp. 2801-2808.
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