Functional vanilloid receptors in cultured normal human epidermal keratinocytes

Kaori Inoue, Schuichi Koizumi, Shigeyoshi Fuziwara, Sumiko Denda, Kazuhide Inoue, Mitsuhiro Denda

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

Vanilloid receptor subtype 1, VR1, is an ion channel that serves as a polymodal detector of pain-producing chemicals such as capsaicin and protons in primary afferent neurons. Here we showed that both capsaicin and acidification produced elevations in the intracellular Ca2+ concentration ([Ca2+]i) in cultured human epidermal keratinocytes. The capsaicin- and acidification-evoked increases in [Ca2+]i were inhibited by capsazepine, an antagonist to VR1. VR1-like immunoreactivity was observed in the cells. These findings suggest that functional VR1-like protein is present and functions as a sensor against noxious chemical stimuli, such as capsaicin or acidification, in epidermal keratinocytes.

Original languageEnglish
Pages (from-to)124-129
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume291
Issue number1
DOIs
Publication statusPublished - Jan 1 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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