Functionalized hydrogel surface patterned in a chip for local PH sensing

H. Maruyama, H. Matsumoto, T. Fukuda, F. Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

9 Citations (Scopus)

Abstract

We developed a novel technique to investigate local pH distribution on a chip using patterned hydrogel film made of UV photosensitive resin. The hydrogel is first patterned by photolithography in any shape on a glass, and later functionalized with the pH indicator, for example, Bromocresol Green (BCG). The hydrogel is polymerized by UV-ray illumination (300-400 nm) on the glass substrate, which is spin coated with the water-soluble prepolymer solution, and immobilized on the glass surface. Unexposed part can be removed easily by water. The hydrogel is hydrophilic, transparent, biocompatible, and is impregnated with the BCG. Then, the Polydimethylsiloxane (PDMS) microchannel is assembled and bonded on the functionalize surface. In this way, we can make patterned pH sensitive surface in the microchannel. Local pH is measured from the color of the hydrogel impregnated with BCG based on the calibrated color information in YCrCb color space. We succeeded in measuring local pH distribution in the microchip.

Original languageEnglish
Title of host publicationMEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems
Pages224-227
Number of pages4
DOIs
Publication statusPublished - 2008
Event21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson - Tucson, AZ, United States
Duration: Jan 13 2008Jan 17 2008

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson
CountryUnited States
CityTucson, AZ
Period1/13/081/17/08

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Maruyama, H., Matsumoto, H., Fukuda, T., & Arai, F. (2008). Functionalized hydrogel surface patterned in a chip for local PH sensing. In MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems (pp. 224-227). [4443633] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2008.4443633